Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. RT-PCR verification of expression for 16 active genes in the ~750-kb CENH3-binding domain. Twelve genes were expressed in all four tissues, one gene (Cen8.t00984) in three tissues, two genes (Cen8.t00985 and Cen8.t01075) in two tissues, and gene Cen8.t00969 in one tissue (calli). Product size matches the prediction for 15 genes, except for gene Cen8.t00891 that yielded a product of 225 bp rather than the predicted 187 bp.
• Supplemental Figure 2 - Supplemental Figure 2. Distribution of genes and repetitive elements within the ~3.5 Mb Cen8 virtual contig. Locations of the 222 annotated genes are depicted as five different coloured boxes indicating the availability of transcriptional evidence. Solid line connects the same repetitive element that is interrupted by other DNA sequences.
• Supplemental Figure 3 - Supplemental Figure 3. Comparison of H3 methylation at Lys 4 (H3K4me2) and H4 acetylation (H4Ac) levels among the five Cen8 domains. The relative fold enrichment (RFE) is calculated for each gene as described in Figure 4. Left panel: CENH3-binding domain A exhibits the highest level of H4 acetylation and slightly low level of H3 methylation at Lys 4. Only RFE values from genes showing methylation or acetylation (α ≤ 0.05 in Student’s t-test) were used to calculate the average RFE (y axis) for each domain. Right panel: CENH3-binding domain A contains similar proportions of genes that show high levels (RFE ≥16) of H3 methylation (Lys 4) and H4 acetylation compared to the other four domains.
• Supplemental Table 1 - Supplemental Table 1. List of genes predicted in the 3.5-Mb Cen8 virtual contig.
• Supplemental Table 2 - Supplemental Table 2. Primers used in RT-PCR and ChIP analysis.
• Supplemental Table 3 - Supplemental Table 3. RT-PCR analysis of Cen8 genes.
• Supplemental Table 4 - Supplemental Table 4. Active genes located in the CENH3-binding domain.