Supplemental Figure 1
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Supplemental Figure 1. Multiple alignments of 11 FT-like genes in rice.
The predicted peptides from FTL/FT-L1(AP002861), Hd3a/FT-L2(AP005828), RFT/FT-L3(AP005858), FT-L4(AP005828), FT-L5(AP004124), FT-L6(OJ991113-30), FT-L7(AL954160), FT-L8(AP003105), FT-L9(AP003076), FT-L10(AP004070) and FT-L11(AP003682) are aligned based on analysis using the CLUSTAL W method.
Supplemental Figure 2
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Supplemental Figure 2. Phytochrome pathway is required for the NB effect.
A. NB (30-min) effect on flowering in WT (Norin 8) and the se5 mutant. Data are mean±s.e.m. of 6-8 plants. B, C, D, Quantification of OsGI, Hd1 and Hd3a mRNAs with 2-h NB in WT (Norin 8) and the se5 mutant. Each mRNA was quantified with ubq mRNA. Data were standardized with 2 separate RNA extractions (mean ± s.e.m). The white and dark bars at the bottom represent the light and dark periods, respectively.
Supplemental Figure 3
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Supplemental Figure 3. OsGI and Hd1 mRNA levels under SD conditions and with 30-min NB in WT (Nipponbare), phyA-2, phyB-1 and phyC-1 mutants.
A, B, C, D, Quantification of OsGI mRNA under SD and with 30-min NB in WT (Nipponbare), phyA-2, phyB-1 and phyC-1, respectively. E, F, G, H, Quantification of Hd1 mRNA under SD and with 30 min NB in WT (Nipponbare), phyA-2, phyB-1 and phyC-1, respectively. Each mRNA was quantified with ubq mRNA. Data were standardized with 2 separate RNA extractions (mean ± s.e.m). The white and dark bars at the bottom represent the light and dark periods, respectively.
Supplemental Figure 4
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Supplemental Figure 4. Quantification of gus mRNA under SD and with 2-h NB in transgenic line 8.
gus mRNA was quantified with ubq mRNA. The white and dark bars at the bottom represent the light and dark periods, respectively.