Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1: ETTIN mediates ectopic KANADI activity in multiple organs. Ectopic expression of KAN2 driven by the AP1 promoter results in filamentous structures taking the place of flowers and growth arrest (A), while in ett-1 AP1>>KAN2 plants (B) all flower organs form and expand (albeit with altered morphology). When KAN2 is driven by the carpel specific CRC promoter, filamentous structures topped with stigmatic papillae form in the place of the gynoecium (C), while in ett-1 CRC>>KAN2 plants the gynoecium is phenotypically indistinguishable from that of ett-1 single mutants(D).
• Supplemental Figure 2 - Supplemental Figure 2: Phenotypic and molecular expression of presumed null ett allele Ectopic expression of KAN1 using the floral specific AP3 promoter results in radial petals (A). AP3:KAN1 ett-13 plants have laterally expanded petals despite ectopic KAN activity (B). Abaxial surface of a kan1-2 kan2-1/+ leaf (C). Abaxial surface of a ett-13 kan1-2 kan2-1/+leaf. Blade outgrowths occur in this background (D), as observed in kan1-2 kan2-1double mutants. (E) Structure of the ETT gene and position of T-DNA insertions in ett-1 andett-13 alleles (Exons boxed and introns marked by lines). 5’ and 3’’ indicate the location of primers that were used for RT-PCR in F. (F) No ETT transcripts beyond T-DNA insertion were detected in ett-1 mutants by RT-PCR analysis, while weak residual amount of truncated transcript was detected in ett-13 mutant background. (G) Changes in floral organ number and variation in various ett mutant alleles are strongly influenced by additional mutation in ARF4 or introduction of truncated ETT transcripts. rp- radial petal, ab- abaxial.