Solution Structure of the B3 DNA Binding Domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1
Plant Cell Yamasaki et al.
16: 3448
Supplemental Data
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Supplemental Figure 1 -
Dependence of the internal energy upon the relative orientation of the component molecules in the model of the RAV1-B3/DNA complex shown in Fig. 4. The helical axis H of the DNA molecule in the complex was rotated around axis P, which passes the gravity center of the protein and the center of the DNA in the contacting region, as schematically shown in the inlet. In each step, the energy was minimized and evaluated by the DISCOVER 3 module in the Insight II molecular modeling package (Accelrys). Note that the energy values of the complex are similar in the range of +/-5 degrees, showing uncertainty of the relative orientation in the model.