Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Regiospecific distribution of fatty acids in triacylglycerols (TAG) from developing seeds of wild-type linseed (24 daf). The positional analysis of fatty acids from TAG were determined as indicated in Methods. Each value is the mean from three independent experiments.
• Supplemental Table 1 - Fatty acid composition of lipid classes of seeds from wild-type and transgenic linseeds. Lipid extracts were prepared from developing seeds (24 daf) of wild-type (A) and transgenic linseed (B). Lipids were fractionated in neutral lipids, phospholipids, and galactolipids, and an aliquot of the different lipid classes was subjected to transesterification and analyzed by GLC as indicated in Methods.
• Supplemental Table 2 - Quantitation of PC species and identification of the acyl anions of the major PC species as a function of the PC mass. ^a PC species are identified in terms of total acyl carbons and total double bonds. In some cases, these identifications may be ambiguous (see footnote c), but chemical formulas corresponding to these identities were used to correct for isotopic overlap of the PC species (Welti et al., 2002). An incorrect chemical formula would cause only a miniscule alteration in the calculated quantities. ^b This footnote indicates that the second species was assigned to the second position on the basis of a stronger signal in the product ion scan. A stronger signal suggests that a species is in the 2-position, although this is not an unequivocal designation of position. ^c These species contained acyl ions of unknown structure. Thus, these ions are indicated by their masses in parentheses.