Supplemental Data

Files in this Data Supplement:

• Supplemental Table 1
• Supplemental Figure 1 - Whole plant ([a] and [c]) and floral ([b] and [d]) phenotype of transgenic plants expressing VIP5 antisense ([a] and [c]) and VIP6 antisense ([b] and [d]) RNA. Plants were grown in inductive (long-day) photoperiods in the absence of a vernalizing cold treatment, and flowered after producing approximately 12 rosette leaves. Bars in each photograph are 2 cm ([a] and [c]) or 1 cm ([b] and [d]).
• Supplemental Figure 2 - Sequence alignment of the predicted VIP6 protein sequence with that of murine p150^TSP and budding yeast Ctr9. The extent of the TPR motifs in VIP6 are overlined with sinuous segments. The predicted protein product of the alternatively spliced VIP6 diverges at amino acid 736, and the sequence of the carboxyl terminus of the resulting truncated protein is shown (-VCHFI*). Similar amino acids are shown in black on grey; identical amino acids are shown in white on black.
• Supplemental Figure 3 - Characterization of VIP4 antisera and VIP3-TAP transgenic plants and antisera. (A) Extracts from flc-3 mutant plants, and from selected vip mutants, were subjected to immunoblot analysis utilizing anti-VIP4 sera. (B) Extracts from flc mutant plants (flc-3), vip3-1 plants containing a transgenic copy of the VIP3-TAP construction, and vip3-1 plants were subjected to immunoblot analysis utilizing anti-VIP3 sera (upper panel) or FLAG antibody (lower panel).
• Supplemental Figure 4 - Sequence alignment of the predicted VIP5 protein with that of budding yeast Rtf1. The extent of the Plus-3 domain is indicated by horizontal brackets. The predicted protein product of the strong vip5-1 allele diverges from that of VIP5 at amino acid 319, and the sequence of the carboxyl terminus of the VIP5-1 protein is shown (-CS*). Shading for conservation of amino acids is as in Supplemental Figure 2.