Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Changes of enzyme activity and transcript levels for each member of the corresponding gene family for Alanine aminotransferase, Aspartate aminotransferase, cyctosolic Fructose bisphosphatase, Fructokinase, Glucokinase, Glucose-6P dehydrogenase, Glutamine synthetase, Glycerokinase, NAD- and NADP-dependent Glyceraldehyde-3P dehydrogenases, Invertase (acid), NADP-dependent Isocitrate dehydrogenase, Phosphoenolpyruvate carboxylase, Pyrophosphate-dependent phosphofructokinase, Pyruvate kinase, Shikimate dehydrogenase, Sucrose-P synthase, and Transketolase in 5-week-old Arabidopsis Col0 wild-type and pgm rosettes throughout a night and day cycle (12 h/12 h) and in wild-type rosettes after a transfer to continuous darkness. The diurnal cycle in wild-type Col0 is shown in the second panel from the left, the diurnal cycle in pgm is shown in the first panel from the left, and the extended night in panels 3 and 4 from the left. Enzyme activities are expressed as nmol g^-1 FW min^-1 +/- SD (n = 5). For nitrate reductase, maximal (max) and selective (sel) activities are given. Transcript levels are expressed as RMA-normalized signals +/- SD (n = 3, wild-type only).
• Supplemental Figure 2 - Examples to illustrate the relations between different randomly generated data sets and the smoothness value (see Methods for the calculation).
• Supplemental Figure 3 - Comparison between Arabidopsis Col0 wild-type (black squares and circles) and pgm (grey squares and circles) of the periodic variation for major transcript (a maximum level >30% of the maximum level of the highest expressed transcript) and summed transcript levels corresponding to the 23 activities studied and for the distribution of their amplitudes. (A) Smoothness classes for transcript levels. (B) Smoothness classes for activities calculated after smoothing. Probability mass function of the amplitudes of transcripts levels (C) and activities (D).
• Supplemental Table 1