Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Total protein extracts, isolated from Arabidopsis thaliana leaves, were separated by SDS-PAGE and blotted to nitrocellulose. Ponceau S staining was first performed to ensure equal protein loading. Preimmune serum used at 1/1000 dilution did not react to any proteins in these extracts. The purified RabA4b antibodies recognized a single band of about 25 kD, correlating with the predicted size of 24.4 kD for RabA4b.
• Supplemental Movie 1 - Treatment with the actin-depolymerizing drug LB causes loss of EYFPRabA4b tip-localization in root hairs. Seedlings expressing EYFP-RabA4b were grown in liquid media and transferred to a perfusion chamber. Images of the root hair were captured every 30 s. Normal root hair expansion was observed for 20 min. Treatment with 200 nM LB resulted in the rapid loss of EYFP-RabA4b tip localization and spreading of the fluorescence along the entire length of the cell. However, upon washout of LB, EYFP-RabA4b reorganized back to the tip of the root hair.