HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef

Supplemental material for “Plant Nuclei Contain Extensive Grooves and Invaginations.”

D.A. Collings, C.N. Carter, J.C. Rink, A.C. Scott, S.E. Wyatt, and N.S. Allen

Videos are submitted in two formats:

*.MOV files are Quicktime animations that can be viewed with the program Quicktime player.

*.AVI files that can be played with the Windows Media Player. (These AVI files can also be played in some versions of the Quicktime player.)

-----------------------

Video 1

 This video accompanies Figure 1 in the text version of the manuscript.

Filename = fig01.mov (2.9 MB) and fig01.avi (4.7 MB)

Time 32.6 seconds

DAPI-Labeling of DNA in an Untransformed Onion Nucleus Demonstrates a Highly Convoluted Nuclear Shape.

(Sequence 1) Confocal optical sections of DAPI fluorescence at 200 nm intervals from the surface of the nucleus. Numerous nuclear grooves (G) score the nuclear surface, while paired nucleoli (N) and a linear invagination (I) lack labeling. The invagination could be followed through all the focal planes, and was visible in transmitted light (not shown in video sequence). Bar = 10 mm.

(Sequence 2) A 3-dimensional reconstruction of DAPI in the nucleus (from Sequence 1) with surface shadowing reveals the convoluted shape of the nucleus, and clearly shows the grooves that score the nuclear surface. Rotation through 180^o shows the nucleus in cross-section roughly at its center, where two nucleoli and the nuclear invagination are seen.

…………………..

Video 2

This video accompanies Figure 4 in the text version of the manuscript.

Filename = fig04.mov (2.3 MB) and fig04.avi (3.4 MB)

Time 35.2 seconds

Imaging  ER-GFP in an Onion Cell Demonstrates the Presence of Nuclear Grooves and Invaginations.

Simultaneous confocal imaging of ER-GFP (green) and DAPI-labeled DNA (blue) in a lens-shaped onion nucleus, with optical sections taken at 200 nm intervals, shows extensive grooving of the nucleus, with ER lying in these grooves. Furthermore, in two locations (red arrows), faint ER channels pass through the center of the nucleus.

-----------------------

Video 3, which accompanies Figure 6 in the text version of the manuscript.

Filename = fig06.mov (3.0 MB) and fig06.avi (2.7 MB)

Time 10 seconds

The Structure of Invaginations and Grooves in Onion Nuclei are Stable.

Simultaneous confocal imaging of ER-GFP (green) and DAPI-labeled DNA (blue) in a single focal plane over time. The sequence represents 44 minutes, and is sped up approximately 400-fold. The nucleus remained in one location during this time, and the position of the two nucleoli (N) remained constant, as did the position of the central nuclear groove. However, nuclear-associated ER showed considerable dynamics during this time. Bar = 10 mm.

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef