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THE PLANT CELL, Vol 9, Issue 4 509-520, Copyright © 1997 by American Society of Plant Biologists
Overexpression of an Endogenous Thionin Enhances Resistance of Arabidopsis against Fusarium oxysporum
P. Epple, K. Apel and H. Bohlmann
Swiss Federal Institute of Technology (ETH), Institute of Plant Sciences, ETH-Zentrum, Universitatstrasse 2, LFW D.58, CH-8092 Zurich, Switzerland
Thionins are antimicrobial proteins that are thought to be involved in
plant defense. Concordant with this view, we have recently shown that the
Arabidopsis thionin Thi2.1 gene is inducible by phytopathogenic fungi.
Here, we demonstrate that constitutive overexpression of this thionin
enhances the resistance of the susceptible ecotype Columbia (Col-2) against
attack by Fusarium oxysporum f sp matthiolae. Transgenic lines had a
reduced loss of chlorophyll after inoculation and supported significantly
less fungal growth on the cotyledons, as evaluated by trypan blue staining.
Moreover, fungi on cotyledons of transgenic lines had more hyphae with
growth anomalies, including hyperbranching, than on cotyledons of the
parental line. No transcripts for pathogenesis-related PR-1, PR-5, or the
pathogen-inducible plant defensin Pdf1.2 could be detected in uninoculated
transgenic seedlings, indicating that all of the observed effects of the
overexpressing lines are most likely the result of the toxicity of the
THI2.1 thionin. Our findings strongly support the view that thionins are
defense proteins.
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