|
THE PLANT CELL, Vol 9, Issue 3 453-462, Copyright © 1997 by American Society of Plant Biologists
A New Class of Plastidic Phosphate Translocators: A Putative Link between Primary and Secondary Metabolism by the Phosphoenolpyruvate/Phosphate Antiporter
K. Fischer, B. Kammerer, M. Gutensohn, B. Arbinger, A. Weber, R. E. Hausler and U. I. Flugge
Botanisches Institut der Universitat zu Koln, Lehrstuhl II, Gyrhofstrasse 15, D-50931 Cologne, Germany
We have purified a plastidic phosphate transport protein from maize
endosperm membranes and cloned and sequenced the corresponding cDNAs from
maize endosperm, maize roots, cauliflower buds, tobacco leaves, and
Arabidopsis leaves. All of these cDNAs exhibit high homology to each other
but only ~30% identity to the known chloroplast triose phosphate/phosphate
translocators. The corresponding genes are expressed in both
photosynthetically active tissues and in nongreen tissues, although
transcripts were more abundant in nongreen tissues. Expression of the
coding region in transformed yeast cells and subsequent transport
measurements of the purified recombinant translocator showed that the
protein mediates transport of inorganic phosphate in exchange with C3
compounds phosphorylated at C-atom 2, particularly phosphoenolpyruvate,
which is required inside the plastids for the synthesis of, for example,
aromatic amino acids. This plastidic phosphate transporter is thus
different in structure and function from the known triose
phosphate/phosphate translocator. We propose that plastids contain various
phosphate translocators with overlapping substrate specificities to ensure
an efficient supply of plastids with a single substrate, even in the
presence of other phosphorylated metabolites.
This article has been cited by other articles:
|
|
|
|
 
M. Linka, A. Jamai, and A. P.M. Weber
Functional Characterization of the Plastidic Phosphate Translocator Gene Family from the Thermo-Acidophilic Red Alga Galdieria sulphuraria Reveals Specific Adaptations of Primary Carbon Partitioning in Green Plants and Red Algae
Plant Physiology,
November 1, 2008;
148(3):
1487 - 1496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Brautigam, S. Hoffmann-Benning, and A. P.M. Weber
Comparative Proteomics of Chloroplast Envelopes from C3 and C4 Plants Reveals Specific Adaptations of the Plastid Envelope to C4 Photosynthesis and Candidate Proteins Required for Maintaining C4 Metabolite Fluxes
Plant Physiology,
September 1, 2008;
148(1):
568 - 579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nozawa, H. Nanamiya, T. Miyata, N. Linka, Y. Endo, A. P. M. Weber, and Y. Tozawa
A Cell-Free Translation and Proteoliposome Reconstitution System for Functional Analysis of Plant Solute Transporters
Plant Cell Physiol.,
December 1, 2007;
48(12):
1815 - 1820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fleige, K. Fischer, D. J. P. Ferguson, U. Gross, and W. Bohne
Carbohydrate Metabolism in the Toxoplasma gondii Apicoplast: Localization of Three Glycolytic Isoenzymes, the Single Pyruvate Dehydrogenase Complex, and a Plastid Phosphate Translocator
Eukaryot. Cell,
June 1, 2007;
6(6):
984 - 996.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Ding, D. Hofius, M.-R. Hajirezaei, A. R. Fernie, F. Bornke, and U. Sonnewald
Functional analysis of the essential bifunctional tobacco enzyme 3-dehydroquinate dehydratase/shikimate dehydrogenase in transgenic tobacco plants
J. Exp. Bot.,
June 1, 2007;
58(8):
2053 - 2067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Bowsher, A. E. Lacey, G. T. Hanke, D. T. Clarkson, L. R. Saker, I. Stulen, and M. J. Emes
The effect of Glc6P uptake and its subsequent oxidation within pea root plastids on nitrite reduction and glutamate synthesis
J. Exp. Bot.,
March 1, 2007;
58(5):
1109 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bouvier, N. Linka, J.-C. Isner, J. Mutterer, A. P.M. Weber, and B. Camara
Arabidopsis SAMT1 Defines a Plastid Transporter Regulating Plastid Biogenesis and Plant Development
PLANT CELL,
November 1, 2006;
18(11):
3088 - 3105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Noutoshi, T. Ito, and K. Shinozaki
ALBINO AND PALE GREEN 10 Encodes BBMII Isomerase Involved in Histidine Biosynthesis in Arabidopsis thaliana
Plant Cell Physiol.,
July 1, 2005;
46(7):
1165 - 1172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Leroch, S. Kirchberger, I. Haferkamp, M. Wahl, H. E. Neuhaus, and J. Tjaden
Identification and Characterization of a Novel Plastidic Adenine Nucleotide Uniporter from Solanum tuberosum
J. Biol. Chem.,
May 6, 2005;
280(18):
17992 - 18000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Niewiadomski, S. Knappe, S. Geimer, K. Fischer, B. Schulz, U. S. Unte, M. G. Rosso, P. Ache, U.-I. Flugge, and A. Schneider
The Arabidopsis Plastidic Glucose 6-Phosphate/Phosphate Translocator GPT1 Is Essential for Pollen Maturation and Embryo Sac Development
PLANT CELL,
March 1, 2005;
17(3):
760 - 775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bakker, F. Routier, S. Oelmann, W. Jordi, A. Lommen, R. Gerardy-Schahn, and D. Bosch
Molecular cloning of two Arabidopsis UDP-galactose transporters by complementation of a deficient Chinese hamster ovary cell line
Glycobiology,
February 1, 2005;
15(2):
193 - 201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Sriram, D. B. Fulton, V. V. Iyer, J. M. Peterson, R. Zhou, M. E. Westgate, M. H. Spalding, and J. V. Shanks
Quantification of Compartmented Metabolic Fluxes in Developing Soybean Embryos by Employing Biosynthetically Directed Fractional 13C Labeling, Two-Dimensional [13C, 1H] Nuclear Magnetic Resonance, and Comprehensive Isotopomer Balancing
Plant Physiology,
October 1, 2004;
136(2):
3043 - 3057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Kubis, M. J. Pike, C. J. Everett, L. M. Hill, and S. Rawsthorne
The import of phosphoenolpyruvate by plastids from developing embryos of oilseed rape, Brassica napus (L.), and its potential as a substrate for fatty acid synthesis
J. Exp. Bot.,
July 1, 2004;
55(402):
1455 - 1462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. M. Weber, J. Schneidereit, and L. M. Voll
Using mutants to probe the in vivo function of plastid envelope membrane metabolite transporters
J. Exp. Bot.,
June 1, 2004;
55(400):
1231 - 1244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Knappe, U.-I. Flugge, and K. Fischer
Analysis of the Plastidic phosphate translocator Gene Family in Arabidopsis and Identification of New phosphate translocator-Homologous Transporters, Classified by Their Putative Substrate-Binding Site
Plant Physiology,
March 1, 2003;
131(3):
1178 - 1190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J.K. Koo and J. B. Ohlrogge
The Predicted Candidates of Arabidopsis Plastid Inner Envelope Membrane Proteins and Their Expression Profiles
Plant Physiology,
October 1, 2002;
130(2):
823 - 836.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. K. Versaw and M. J. Harrison
A Chloroplast Phosphate Transporter, PHT2;1, Influences Allocation of Phosphate within the Plant and Phosphate-Starvation Responses
PLANT CELL,
August 1, 2002;
14(8):
1751 - 1766.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Hausler, H.-J. Hirsch, F. Kreuzaler, and C. Peterhansel
Overexpression of C4-cycle enzymes in transgenic C3 plants: a biotechnological approach to improve C3-photosynthesis
J. Exp. Bot.,
April 1, 2002;
53(369):
591 - 607.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Eicks, V. Maurino, S. Knappe, U.-I. Flugge, and K. Fischer
The Plastidic Pentose Phosphate Translocator Represents a Link between the Cytosolic and the Plastidic Pentose Phosphate Pathways in Plants
Plant Physiology,
February 1, 2002;
128(2):
512 - 522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Farre, A. Tiessen, U. Roessner, P. Geigenberger, R. N. Trethewey, and L. Willmitzer
Analysis of the Compartmentation of Glycolytic Intermediates, Nucleotides, Sugars, Organic Acids, Amino Acids, and Sugar Alcohols in Potato Tubers Using a Nonaqueous Fractionation Method
Plant Physiology,
October 1, 2001;
127(2):
685 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Hausler, T. Rademacher, J. Li, V. Lipka, K. L. Fischer, S. Schubert, F. Kreuzaler, and H.-J. Hirsch
Single and double overexpression of C4-cycle genes had differential effects on the pattern of endogenous enzymes, attenuation of photorespiration and on contents of UV protectants in transgenic potato and tobacco plants
J. Exp. Bot.,
September 1, 2001;
52(362):
1785 - 1803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Bowsher and A. K. Tobin
Compartmentation of metabolism within mitochondria and plastids
J. Exp. Bot.,
April 1, 2001;
52(356):
513 - 527.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Henkes, U. Sonnewald, R. Badur, R. Flachmann, and M. Stitt
A Small Decrease of Plastid Transketolase Activity in Antisense Tobacco Transformants Has Dramatic Effects on Photosynthesis and Phenylpropanoid Metabolism
PLANT CELL,
March 1, 2001;
13(3):
535 - 551.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. A. White, J. Todd, T. Newman, N. Focks, T. Girke, O. M. de Ilárduya, J. G. Jaworski, J. B. Ohlrogge, and C. Benning
A New Set of Arabidopsis Expressed Sequence Tags from Developing Seeds. The Metabolic Pathway from Carbohydrates to Seed Oil
Plant Physiology,
December 1, 2000;
124(4):
1582 - 1594.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Suzuki, N. Murai, J. N. Burnell, and M. Arai
Changes in Photosynthetic Carbon Flow in Transgenic Rice Plants That Express C4-Type Phosphoenolpyruvate Carboxykinase from Urochloa panicoides
Plant Physiology,
September 1, 2000;
124(1):
163 - 172.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. J. Streatfield, A. Weber, E. A. Kinsman, R. E. Häusler, J. Li, D. Post-Beittenmiller, W. M. Kaiser, K. A. Pyke, U.-I. Flügge, and J. Chory
The Phosphoenolpyruvate/Phosphate Translocator Is Required for Phenolic Metabolism, Palisade Cell Development, and Plastid-Dependent Nuclear Gene Expression
PLANT CELL,
September 1, 1999;
11(9):
1609 - 1622.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. C. Shannon, F.-M. Pien, H. Cao, and K.-C. Liu
Brittle-1, an Adenylate Translocator, Facilitates Transfer of Extraplastidial Synthesized ADP-Glucose into Amyloplasts of Maize Endosperms
Plant Physiology,
August 1, 1998;
117(4):
1235 - 1252.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Pohlmeyer, J. Soll, R. Grimm, K. Hill, and R. Wagner
A High-Conductance Solute Channel in the Chloroplastic Outer Envelope from Pea
PLANT CELL,
July 1, 1998;
10(7):
1207 - 1216.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Kammerer, K. Fischer, B. Hilpert, S. Schubert, M. Gutensohn, A. Weber, and U.-I. Flügge
Molecular Characterization of a Carbon Transporter in Plastids from Heterotrophic Tissues: The Glucose 6-Phosphate/Phosphate Antiporter
PLANT CELL,
January 1, 1998;
10(1):
105 - 118.
[Abstract]
[Full Text]
|
|
|
|
|
