Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (60)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nam, J.
Right arrow Articles by Gelvin, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nam, J.
Right arrow Articles by Gelvin, S. B.
Agricola
Right arrow Articles by Nam, J.
Right arrow Articles by Gelvin, S. B.

THE PLANT CELL, Vol 9, Issue 3 317-333, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

Differences in Susceptibility of Arabidopsis Ecotypes to Crown Gall Disease May Result from a Deficiency in T-DNA Integration

J. Nam, A. G. Matthysse and S. B. Gelvin
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392

We show that among ecotypes of Arabidopsis, there is considerable variation in their susceptibility to crown gall disease. Differences in susceptibility are heritable and, in one ecotype, segregate as a single major contributing locus. In several ecotypes, recalcitrance to tumorigenesis results from decreased binding of Agrobacterium to inoculated root explants. The recalcitrance of another ecotype occurs at a late step in T-DNA transfer. Transient expression of a T-DNA-encoded [beta]-glucuronidase gusA gene is efficient, but the ecotype is deficient in crown gall tumorigenesis, transformation to kanamycin resistance, and stable GUS expression. This ecotype is also more sensitive to [gamma] radiation than is a susceptible ecotype. DNA gel blot analysis showed that after infection by Agrobacterium, less T-DNA was integrated into the genome of the recalcitrant ecotype than was integrated into the genome of a highly susceptible ecotype.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
L.-Y. Lee, M. E. Kononov, B. Bassuner, B. R. Frame, K. Wang, and S. B. Gelvin
Novel Plant Transformation Vectors Containing the Superpromoter
Plant Physiology, December 1, 2007; 145(4): 1294 - 1300.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. M. Crane and S. B. Gelvin
RNAi-mediated gene silencing reveals involvement of Arabidopsis chromatin-related genes in Agrobacterium-mediated root transformation
PNAS, September 18, 2007; 104(38): 15156 - 15161.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Abe, K. Osakabe, S. Nakayama, M. Endo, A. Tagiri, S. Todoriki, H. Ichikawa, and S. Toki
Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis
Plant Physiology, October 1, 2005; 139(2): 896 - 908.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H.-H. Hwang and S. B. Gelvin
Plant Proteins That Interact with VirB2, the Agrobacterium tumefaciens Pilin Protein, Mediate Plant Transformation
PLANT CELL, November 1, 2004; 16(11): 3148 - 3167.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Tao, P. K. Rao, S. Bhattacharjee, and S. B. Gelvin
Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium T-complex protein VirD2
PNAS, April 6, 2004; 101(14): 5164 - 5169.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Lee, J. L. Humann, J. S. Pitrak, J. T. Cuperus, T. D. Parks, C. A. Whistler, M. C. Mok, and L. W. Ream
Translation Start Sequences Affect the Efficiency of Silencing of Agrobacterium tumefaciens T-DNA Oncogenes
Plant Physiology, November 1, 2003; 133(3): 966 - 977.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Zhu, J. Nam, J. M. Humara, K. S. Mysore, L.-Y. Lee, H. Cao, L. Valentine, J. Li, A. D. Kaiser, A. L. Kopecky, et al.
Identification of Arabidopsis rat Mutants
Plant Physiology, June 1, 2003; 132(2): 494 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
S. B. Gelvin
Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool
Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 16 - 37.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Escobar, E. L. Civerolo, K. R. Summerfelt, and A. M. Dandekar
RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis
PNAS, October 25, 2001; (2001) 241276898.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Chateau, R. S. Sangwan, and B. S. Sangwan-Norreel
Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer: role of phytohormones
J. Exp. Bot., December 1, 2000; 51(353): 1961 - 1968.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. M. Bravo-Angel, V. Gloeckler, B. Hohn, and B. Tinland
Bacterial Conjugation Protein MobA Mediates Integration of Complex DNA Structures into Plant Cells
J. Bacteriol., September 15, 1999; 181(18): 5758 - 5765.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
L.-Y. Lee, S. B. Gelvin, and C. I. Kado
pSa Causes Oncogenic Suppression of Agrobacterium by Inhibiting VirE2 Protein Export
J. Bacteriol., January 1, 1999; 181(1): 186 - 196.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
A. G. Matthysse and S. McMahan
Root Colonization by Agrobacterium tumefaciens Is Reduced in cel, attB, attD, and attR Mutants
Appl. Envir. Microbiol., July 1, 1998; 64(7): 2341 - 2345.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Kohli, M. Leech, P. Vain, D. A. Laurie, and P. Christou
Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots
PNAS, June 9, 1998; 95(12): 7203 - 7208.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Escobar, E. L. Civerolo, K. R. Summerfelt, and A. M. Dandekar
RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis
PNAS, November 6, 2001; 98(23): 13437 - 13442.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1997 by the American Society of Plant Biologists