Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (72)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jenkins, B. D.
Right arrow Articles by Barkan, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jenkins, B. D.
Right arrow Articles by Barkan, A.
Agricola
Right arrow Articles by Jenkins, B. D.
Right arrow Articles by Barkan, A.

THE PLANT CELL, Vol 9, Issue 3 283-296, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

Nuclear Mutations That Block Group II RNA Splicing in Maize Chloroplasts Reveal Several Intron Classes with Distinct Requirements for Splicing Factors

B. D. Jenkins, D. J. Kulhanek and A. Barkan
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229

To elucidate mechanisms that regulate chloroplast RNA splicing in multicellular plants, we sought nuclear mutations in maize that result in chloroplast splicing defects. Evidence is presented for two nuclear genes whose function is required for the splicing of group II introns in maize chloroplasts. A mutation in the crs1 (for chloroplast RNA splicing 1) gene blocks the splicing of only the atpF intron, whereas a mutation in the crs2 gene blocks the splicing of many chloroplast introns. In addition, a correlation was observed between the absence of plastid ribosomes and the failure to splice several chloroplast introns. Our results suggest that a chloroplast-encoded factor and a nuclear-encoded factor whose activity requires crs2 function facilitate the splicing of distinct sets of group II introns. These two genetically defined intron sets also differ with regard to intron structure: one set consists of only subgroup IIA introns and the other of only subgroup IIB introns. Therefore, it is likely that distinct splicing factors recognize subgroup-specific features of intron structure or facilitate subgroup-specific aspects of the splicing reaction. Of the 12 pre-mRNA introns in the maize chloroplast genome, only one is normally spliced in both crs2 mutants and in mutants lacking plastid ribosomes, indicating that few, if any, of the group II introns in the chloroplast genome undergo autocatalytic splicing in vivo.


This article has been cited by other articles:


Home page
 RNAHome page
Y. Asakura, O. A. Bayraktar, and A. Barkan
Two CRM protein subfamilies cooperate in the splicing of group IIB introns in chloroplasts
RNA, November 1, 2008; 14(11): 2319 - 2332.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Beick, C. Schmitz-Linneweber, R. Williams-Carrier, B. Jensen, and A. Barkan
The Pentatricopeptide Repeat Protein PPR5 Stabilizes a Specific tRNA Precursor in Maize Chloroplasts
Mol. Cell. Biol., September 1, 2008; 28(17): 5337 - 5347.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Prikryl, K. P. Watkins, G. Friso, K. J. van Wijk, and A. Barkan
A member of the Whirly family is a multifunctional RNA- and DNA-binding protein that is essential for chloroplast biogenesis
Nucleic Acids Res., September 1, 2008; 36(16): 5152 - 5165.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Keren, L. Klipcan, A. Bezawork-Geleta, M. Kolton, F. Shaya, and O. Ostersetzer-Biran
Characterization of the Molecular Basis of Group II Intron RNA Recognition by CRS1-CRM Domains
J. Biol. Chem., August 22, 2008; 283(34): 23333 - 23342.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Kahlau and R. Bock
Plastid Transcriptomics and Translatomics of Tomato Fruit Development and Chloroplast-to-Chromoplast Differentiation: Chromoplast Gene Expression Largely Serves the Production of a Single Protein
PLANT CELL, April 1, 2008; 20(4): 856 - 874.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
N. Bolle, I. Hinrichsen, and F. Kempken
Plastid mRNAs are neither spliced nor edited in maize and cauliflower mitochondrial in organello systems
RNA, December 1, 2007; 13(12): 2061 - 2065.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Asakura and A. Barkan
A CRM Domain Protein Functions Dually in Group I and Group II Intron Splicing in Land Plant Chloroplasts
PLANT CELL, December 1, 2007; 19(12): 3864 - 3875.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
M. M. Barthet and K. W. Hilu
Expression of matK: functional and evolutionary implications
Am. J. Botany, August 1, 2007; 94(8): 1402 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. P. Watkins, T. S. Kroeger, A. M. Cooke, R. E. Williams-Carrier, G. Friso, S. E. Belcher, K. J. van Wijk, and A. Barkan
A Ribonuclease III Domain Protein Functions in Group II Intron Splicing in Maize Chloroplasts
PLANT CELL, August 1, 2007; 19(8): 2606 - 2623.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Asakura and A. Barkan
Arabidopsis Orthologs of Maize Chloroplast Splicing Factors Promote Splicing of Orthologous and Species-Specific Group II Introns
Plant Physiology, December 1, 2006; 142(4): 1656 - 1663.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Schmitz-Linneweber, R. E. Williams-Carrier, P. M. Williams-Voelker, T. S. Kroeger, A. Vichas, and A. Barkan
A Pentatricopeptide Repeat Protein Facilitates the trans-Splicing of the Maize Chloroplast rps12 Pre-mRNA
PLANT CELL, October 1, 2006; 18(10): 2650 - 2663.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. J. Ostheimer, M. Rojas, H. Hadjivassiliou, and A. Barkan
Formation of the CRS2-CAF2 Group II Intron Splicing Complex Is Mediated by a 22-Amino Acid Motif in the COOH-terminal Region of CAF2
J. Biol. Chem., February 24, 2006; 281(8): 4732 - 4738.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
G. Hausner, R. Olson, D. Simon, I. Johnson, E. R. Sanders, K. G. Karol, R. M. McCourt, and S. Zimmerly
Origin and Evolution of the Chloroplast trnK (matK) Intron: A Model for Evolution of Group II Intron RNA Structures
Mol. Biol. Evol., February 1, 2006; 23(2): 380 - 391.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
O. Ostersetzer, A. M. Cooke, K. P. Watkins, and A. Barkan
CRS1, a Chloroplast Group II Intron Splicing Factor, Promotes Intron Folding through Specific Interactions with Two Intron Domains
PLANT CELL, January 1, 2005; 17(1): 241 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Sugimoto, K. Kusumi, Y. Tozawa, J. Yazaki, N. Kishimoto, S. Kikuchi, and K. Iba
The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an Early Stage of Chloroplast Differentiation
Plant Cell Physiol., August 15, 2004; 45(8): 985 - 996.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
O. W. ODOM, D. L. SHENKENBERG, J. A. GARCIA, and D. L. HERRIN
A horizontally acquired group II intron in the chloroplast psbA gene of a psychrophilic Chlamydomonas: In vitro self-splicing and genetic evidence for maturase activity
RNA, July 1, 2004; 10(7): 1097 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. PERRON, M. GOLDSCHMIDT-CLERMONT, and J.-D. ROCHAIX
A multiprotein complex involved in chloroplast group II intron splicing
RNA, April 1, 2004; 10(4): 704 - 711.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Meierhoff, S. Felder, T. Nakamura, N. Bechtold, and G. Schuster
HCF152, an Arabidopsis RNA Binding Pentatricopeptide Repeat Protein Involved in the Processing of Chloroplast psbB-psbT-psbH-petB-petD RNAs
PLANT CELL, June 1, 2003; 15(6): 1480 - 1495.
[Abstract] [Full Text]

Home page
 Plant CellHome page
K. Lennartz, H. Plucken, A. Seidler, P. Westhoff, N. Bechtold, and K. Meierhoff
HCF164 Encodes a Thioredoxin-Like Protein Involved in the Biogenesis of the Cytochrome b6f Complex in Arabidopsis
PLANT CELL, November 1, 2001; 13(11): 2539 - 2551.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. E. Vaistij, E. Boudreau, S. D. Lemaire, M. Goldschmidt-Clermont, and J.-D. Rochaix
Characterization of Mbb1, a nucleus-encoded tetratricopeptide-like repeat protein required for expression of the chloroplast psbB/psbT/psbH gene cluster in Chlamydomonas reinhardtii
PNAS, December 19, 2000; 97(26): 14813 - 14818.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. M. d. Campo, B. Sabater, and M. Martin
Transcripts of the ndhH-D operon of barley plastids: possible role of unedited site III in splicing of the ndhA intron
Nucleic Acids Res., March 1, 2000; 28(5): 1092 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Levy, K. L. Kindle, and D. B. Stern
Target and Specificity of a Nuclear Gene Product That Participates in mRNA 3'-End Formation in Chlamydomonas Chloroplasts
J. Biol. Chem., December 10, 1999; 274(50): 35955 - 35962.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. C. Higgs, R. S. Shapiro, K. L. Kindle, and D. B. Stern
Small cis-Acting Sequences That Specify Secondary Structures in a Chloroplast mRNA Are Essential for RNA Stability and Translation
Mol. Cell. Biol., December 1, 1999; 19(12): 8479 - 8491.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Vogel, T. Borner, and W. R. Hess
Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants
Nucleic Acids Res., October 1, 1999; 27(19): 3866 - 3874.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Schuenemann, P. Amin, E. Hartmann, and N. E. Hoffman
Chloroplast SecY Is Complexed to SecE and Involved in the Translocation of the 33-kDa but Not the 23-kDa Subunit of the Oxygen-evolving Complex
J. Biol. Chem., April 23, 1999; 274(17): 12177 - 12182.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. M. Roy and A. Barkan
A SecY Homologue Is Required for the Elaboration of the Chloroplast Thylakoid Membrane and for Normal Chloroplast Gene Expression
J. Cell Biol., April 20, 1998; 141(2): 385 - 395.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nakamura, M. Ohta, M. Sugiura, and M. Sugita
Chloroplast Ribonucleoproteins Function as a Stabilizing Factor of Ribosome-free mRNAs in the Stroma
J. Biol. Chem., January 5, 2001; 276(1): 147 - 152.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. McCormac, H. Litz, J. Wang, P. D. Gollnick, and J. O. Berry
Light-associated and Processing-dependent Protein Binding to 5' Regions of rbcL mRNA in the Chloroplasts of a C4 Plant
J. Biol. Chem., January 26, 2001; 276(5): 3476 - 3483.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1997 by the American Society of Plant Biologists