Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (67)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, M. S.
Right arrow Articles by Trelease, R. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, M. S.
Right arrow Articles by Trelease, R. N.
Agricola
Right arrow Articles by Lee, M. S.
Right arrow Articles by Trelease, R. N.

THE PLANT CELL, Vol 9, Issue 2 185-197, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

Oilseed Isocitrate Lyases Lacking Their Essential Type 1 Peroxisomal Targeting Signal Are Piggybacked to Glyoxysomes

M. S. Lee, R. T. Mullen and R. N. Trelease
Department of Botany and Graduate Program in Molecular and Cellular Biology, Arizona State University, Tempe, Arizona 85287-1601

Isocitrate lyase (IL) is an essential enzyme in the glyoxylate cycle, which is a pathway involved in the mobilization of stored lipids during postgerminative growth of oil-rich seedlings. We determined experimentally the necessary and sufficient peroxisome targeting signals (PTSs) for cottonseed, oilseed rape, and castor bean ILs in a well-characterized in vivo import system, namely, suspension-cultured tobacco (Bright Yellow) BY-2 cells. Results were obtained by comparing immunofluorescence localizations of wild-type and C-terminal-truncated proteins transiently expressed from cDNAs introduced by microprojectile bombardment. The tripeptides ARM-COOH (on cottonseed and castor bean ILs) and SRM-COOH (on oilseed rape IL) were necessary for targeting and actual import of these ILs into glyoxysomes, and ARM-COOH was sufficient for redirecting chloramphenicol acetyltransferase (CAT) from the cytosol into the glyoxysomes. Surprisingly, IL and CAT subunits without these tripeptides were still acquired by glyoxysomes, but only when wild-type IL or CAT-SKL subunits, respectively, were simultaneously expressed in the cells. These results reveal that targeting signal-depleted subunits are being piggybacked as multimers to glyoxysomes by association with subunits possessing a PTS1. Targeted multimers are then translocated through membrane pores or channels to the matrix as oligomers or as subunits before reoligomerization in the matrix.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
T. Flores, C. D. Todd, A. Tovar-Mendez, P. K. Dhanoa, N. Correa-Aragunde, M. E. Hoyos, D. M. Brownfield, R. T. Mullen, L. Lamattina, and J. C. Polacco
Arginase-Negative Mutants of Arabidopsis Exhibit Increased Nitric Oxide Signaling in Root Development
Plant Physiology, August 1, 2008; 147(4): 1936 - 1946.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. P. Simpson, R. Di Leo, P. K. Dhanoa, W. L. Allan, A. Makhmoudova, S. M. Clark, G. J. Hoover, R. T. Mullen, and B. J. Shelp
Identification and characterization of a plastid-localized Arabidopsis glyoxylate reductase isoform: comparison with a cytosolic isoform and implications for cellular redox homeostasis and aldehyde detoxification
J. Exp. Bot., June 1, 2008; 59(9): 2545 - 2554.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. J. Lingard, S. K. Gidda, S. Bingham, S. J. Rothstein, R. T. Mullen, and R. N. Trelease
Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle-Associated Replication of Peroxisomes
PLANT CELL, June 1, 2008; 20(6): 1567 - 1585.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. K. Karnik and R. N. Trelease
Arabidopsis peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals
J. Exp. Bot., May 1, 2007; 58(7): 1677 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. K. Karnik and R. N. Trelease
Arabidopsis Peroxin 16 Coexists at Steady State in Peroxisomes and Endoplasmic Reticulum
Plant Physiology, August 1, 2005; 138(4): 1967 - 1981.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. T. Hwang, S. M. Pelitire, M. P.A. Henderson, D. W. Andrews, J. M. Dyer, and R. T. Mullen
Novel Targeting Signals Mediate the Sorting of Different Isoforms of the Tail-Anchored Membrane Protein Cytochrome b5 to Either Endoplasmic Reticulum or Mitochondria
PLANT CELL, November 1, 2004; 16(11): 3002 - 3019.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G.-W. Tian, A. Mohanty, S. N. Chary, S. Li, B. Paap, G. Drakakaki, C. D. Kopec, J. Li, D. Ehrhardt, D. Jackson, et al.
High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta
Plant Physiology, May 1, 2004; 135(1): 25 - 38.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. L. Johnson and L. J. Olsen
Import of the Peroxisomal Targeting Signal Type 2 Protein 3-Ketoacyl-Coenzyme A Thiolase into Glyoxysomes
Plant Physiology, December 1, 2003; 133(4): 1991 - 1999.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
C. S. Lisenbee, M. Heinze, and R. N. Trelease
Peroxisomal Ascorbate Peroxidase Resides within a Subdomain of Rough Endoplasmic Reticulum in Wild-Type Arabidopsis Cells
Plant Physiology, June 1, 2003; 132(2): 870 - 882.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. L. Stone, E. M. Anderson, R. T. Mullen, and D. R. Goring
ARC1 Is an E3 Ubiquitin Ligase and Promotes the Ubiquitination of Proteins during the Rejection of Self-Incompatible Brassica Pollen
PLANT CELL, April 1, 2003; 15(4): 885 - 898.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. T. J. Klein, M. van den Berg, G. Bottger, H. F. Tabak, and B. Distel
Saccharomyces cerevisiae Acyl-CoA Oxidase Follows a Novel, Non-PTS1, Import Pathway into Peroxisomes That Is Dependent on Pex5p
J. Biol. Chem., July 5, 2002; 277(28): 25011 - 25019.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Nito, M. Hayashi, and M. Nishimura
Direct Interaction and Determination of Binding Domains among Peroxisomal Import Factors in Arabidopsis thaliana
Plant Cell Physiol., April 15, 2002; 43(4): 355 - 366.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
H. Horiguchi, H. Yurimoto, T.-K. Goh, T. Nakagawa, N. Kato, and Y. Sakai
Peroxisomal Catalase in the Methylotrophic Yeast Candida boidinii: Transport Efficiency and Metabolic Significance
J. Bacteriol., November 1, 2001; 183(21): 6372 - 6383.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
L. M. Olivier, W. Kovacs, K. Masuda, G.-A. Keller, and S. K. Krisans
Identification of peroxisomal targeting signals in cholesterol biosynthetic enzymes: AA-CoA thiolase, HMG-CoA synthase, MPPD, and FPP synthase
J. Lipid Res., December 1, 2000; 41(12): 1921 - 1935.
[Abstract] [Full Text]

Home page
 Plant CellHome page
R. T. Mullen, C. S. Lisenbee, J. A. Miernyk, and R. N. Trelease
Peroxisomal Membrane Ascorbate Peroxidase Is Sorted to a Membranous Network That Resembles a Subdomain of the Endoplasmic Reticulum
PLANT CELL, November 1, 1999; 11(11): 2167 - 2186.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. J. Lumb, A. F. Drake, and C. J. Danpure
Effect of N-terminal alpha -Helix Formation on the Dimerization and Intracellular Targeting of Alanine:Glyoxylate Aminotransferase
J. Biol. Chem., July 16, 1999; 274(29): 20587 - 20596.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
V. I. Titorenko, J. J. Smith, R. K. Szilard, and R. A. Rachubinski
Pex20p of the Yeast Yarrowia lipolytica Is Required for the Oligomerization of Thiolase in the Cytosol and for Its Targeting to the Peroxisome
J. Cell Biol., July 27, 1998; 142(2): 403 - 420.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. J. Crookes and L. J. Olsen
The Effects of Chaperones and the Influence of Protein Assembly on Peroxisomal Protein Import
J. Biol. Chem., July 3, 1998; 273(27): 17236 - 17242.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. A. Teter and S. M. Theg
Energy-transducing thylakoid membranes remain highly impermeable to ions during protein translocation
PNAS, February 17, 1998; 95(4): 1590 - 1594.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. SUBRAMANI
Components Involved in Peroxisome Import, Biogenesis, Proliferation, Turnover, and Movement
Physiol Rev, January 1, 1998; 78(1): 171 - 188.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. T. Mullen and R. N. Trelease
The Sorting Signals for Peroxisomal Membrane-bound Ascorbate Peroxidase Are within Its C-terminal Tail
J. Biol. Chem., May 19, 2000; 275(21): 16337 - 16344.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
V. I. Titorenko, J.-M. Nicaud, H. Wang, H. Chan, and R. A. Rachubinski
Acyl-CoA oxidase is imported as a heteropentameric, cofactor-containing complex into peroxisomes of Yarrowia lipolytica
J. Cell Biol., February 4, 2002; 156(3): 481 - 494.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Subramani
Hitchhiking fads en route to peroxisomes
J. Cell Biol., February 4, 2002; 156(3): 415 - 417.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1997 by the American Society of Plant Biologists