Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (62)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Albani, D.
Right arrow Articles by Bevan, M. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Albani, D.
Right arrow Articles by Bevan, M. W.
Agricola
Right arrow Articles by Albani, D.
Right arrow Articles by Bevan, M. W.

THE PLANT CELL, Vol 9, Issue 2 171-184, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

The Wheat Transcriptional Activator SPA: A Seed-Specific bZIP Protein That Recognizes the GCN4-like Motif in the Bifactorial Endosperm Box of Prolamin Genes

D. Albani, MCU. Hammond-Kosack, C. Smith, S. Conlan, V. Colot, M. Holdsworth and M. W. Bevan
Molecular Genetics Department, John Innes Centre, Colney Lane, Norwich NR4 7UJ, United Kingdom

The conserved bifactorial endosperm box found in the promoter of wheat storage protein genes comprises two different cis elements that are thought to be involved in regulating endosperm-specific gene expression. Endosperm nuclear extracts contain binding activities. One is called ESBF-I, which binds to the endosperm motif (EM), and the other is called ESBF-II, which binds to the GCN4-like motif (GLM). Here, we present a functional analysis of the endosperm box of a low-molecular-weight glutenin gene found on the 1D1 chromosome of hexaploid wheat (LMWG-1D1) in transgenic tobacco plants. Our analysis demonstrates the necessity of the EM and GLM for endosperm-specific gene expression and suggests the presence in tobacco of functional counterparts of wheat ESBF-I and ESBF-II. Furthermore, we describe the isolation and characterization of cDNA clones encoding SPA, a seed-specific basic leucine zipper protein from wheat that can activate transcription from the GLMs of the -326-bp LMWG-1D1 promoter in both maize and tobacco leaf protoplasts. This activation is also partially dependent on the presence of functional EMs, suggesting interactions between SPA with ESBF-I-like activities.


This article has been cited by other articles:


Home page
 J Exp BotHome page
T. Kawakatsu, M. P. Yamamoto, S. Hirose, M. Yano, and F. Takaiwa
Characterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm
J. Exp. Bot., November 2, 2008; (2008) ern265v1.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
T. W. J. M. Van Herpen, M. Riley, C. Sparks, H. D. Jones, C. Gritsch, E. H. Dekking, R. J. Hamer, D. Bosch, E. M. J. Salentijn, M. J. M. Smulders, et al.
Detailed Analysis of the Expression of an Alpha-gliadin Promoter and the Deposition of Alpha-gliadin Protein During Wheat Grain Development
Ann. Bot., September 1, 2008; 102(3): 331 - 342.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. P. Yamamoto, Y. Onodera, S. M. Touno, and F. Takaiwa
Synergism between RPBF Dof and RISBZ1 bZIP Activators in the Regulation of Rice Seed Expression Genes
Plant Physiology, August 1, 2006; 141(4): 1694 - 1707.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y.-S. Hwang, P. Ciceri, R. L. Parsons, S. P. Moose, R. J. Schmidt, and N. Huang
The Maize O2 and PBF Proteins Act Additively to Promote Transcription from Storage Protein Gene Promoters in Rice Endosperm Cells
Plant Cell Physiol., October 15, 2004; 45(10): 1509 - 1518.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Yanagisawa
Dof Domain Proteins: Plant-Specific Transcription Factors Associated with Diverse Phenomena Unique to Plants
Plant Cell Physiol., April 15, 2004; 45(4): 386 - 391.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. Satoh, Y. Fujita, K. Nakashima, K. Shinozaki, and K. Yamaguchi-Shinozaki
A Novel Subgroup of bZIP Proteins Functions as Transcriptional Activators in Hypoosmolarity-Responsive Expression of the ProDH Gene in Arabidopsis
Plant Cell Physiol., March 15, 2004; 45(3): 309 - 317.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Lara, L. Onate-Sanchez, Z. Abraham, C. Ferrandiz, I. Diaz, P. Carbonero, and J. Vicente-Carbajosa
Synergistic Activation of Seed Storage Protein Gene Expression in Arabidopsis by ABI3 and Two bZIPs Related to OPAQUE2
J. Biol. Chem., May 30, 2003; 278(23): 21003 - 21011.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. R. Shewry and N. G. Halford
Cereal seed storage proteins: structures, properties and role in grain utilization
J. Exp. Bot., April 15, 2002; 53(370): 947 - 958.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Mariconti, B. Pellegrini, R. Cantoni, R. Stevens, C. Bergounioux, R. Cella, and D. Albani
The E2F Family of Transcription Factors from Arabidopsis thaliana. NOVEL AND CONSERVED COMPONENTS OF THE RETINOBLASTOMA/E2F PATHWAY IN PLANTS
J. Biol. Chem., March 15, 2002; 277(12): 9911 - 9919.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Onate, J. Vicente-Carbajosa, P. Lara, I. Diaz, and P. Carbonero
Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm
J. Biol. Chem., April 2, 1999; 274(14): 9175 - 9182.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. B. Singh
Transcriptional Regulation in Plants: The Importance of Combinatorial Control
Plant Physiology, December 1, 1998; 118(4): 1111 - 1120.
[Full Text]

Home page
 Plant Physiol.Home page
J. X. Zhu-Shimoni and G. Galili
Expression of an Arabidopsis Aspartate Kinase/Homoserine Dehydrogenase Gene Is Metabolically Regulated by Photosynthesis-Related Signals but Not by Nitrogenous Compounds
Plant Physiology, March 1, 1998; 116(3): 1023 - 1028.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Vicente-Carbajosa, S. P. Moose, R. L. Parsons, and R. J. Schmidt
A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2
PNAS, July 8, 1997; 94(14): 7685 - 7690.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Onodera, A. Suzuki, C.-Y. Wu, H. Washida, and F. Takaiwa
A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif
J. Biol. Chem., April 20, 2001; 276(17): 14139 - 14152.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Albani, L. Mariconti, S. Ricagno, L. Pitto, C. Moroni, K. Helin, and R. Cella
DcE2F, a Functional Plant E2F-like Transcriptional Activator from Daucus carota
J. Biol. Chem., June 16, 2000; 275(25): 19258 - 19267.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1997 by the American Society of Plant Biologists