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THE PLANT CELL, Vol 9, Issue 10 1869-1880, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

Protein Quality Control along the Route to the Plant Vacuole

E. Pedrazzini, G. Giovinazzo, A. Bielli, M. de Virgilio, L. Frigerio, M. Pesca, F. Faoro, R. Bollini, A. Ceriotti and A. Vitale
Istituto Biosintesi Vegetali, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milan, Italy

To acquire information on the relationships between structural maturation of proteins in the endoplasmic reticulum (ER) and their transport along the secretory pathway, we have analyzed the destiny of an assembly-defective form of the trimeric vacuolar storage glycoprotein phaseolin. In leaves of transgenic tobacco, where assembly-competent phaseolin is correctly targeted to the vacuole, defective phaseolin remains located in the ER or a closely related compartment where it represents a major ligand of the chaperone BiP. Defective phaseolin maintained susceptibility to endoglycosidase H and was slowly degraded by a process that is not inhibited by heat shock or brefeldin A, indicating that degradation does not involve transport along the secretory pathway. These results provide evidence for the presence of a quality control mechanism in the ER of plant cells that avoids intracellular trafficking of severely defective proteins and eventually leads to their degradation.


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