THE PLANT CELL, Vol 9, Issue 10 1805-1814, Copyright © 1997 by American Society of Plant Biologists

RESEARCH ARTICLE

The 14-3-3 Protein Interacts Directly with the C-Terminal Region of the Plant Plasma Membrane H+-ATPase

T. Jahn, A. T. Fuglsang, A. Olsson, I. M. Bruntrup, D. B. Collinge, D. Volkmann, M. Sommarin, M. G. Palmgren and C. Larsson
Department of Plant Biochemistry, Lund University, P.O. Box 117, S-22100 Lund, Sweden

Accumulating evidence suggests that 14-3-3 proteins are involved in the regulation of plant plasma membrane H+-ATPase activity. However, it is not known whether the 14-3-3 protein interacts directly or indirectly with the H+-ATPase. In this study, detergent-solubilized plasma membrane H+-ATPase isolated from fusicoccin-treated maize shoots was copurified with the 14-3-3 protein (as determined by protein gel blotting), and the H+-ATPase was recovered in an activated state. In the absence of fusicoccin treatment, H +-ATPase and the 14-3-3 protein were well separated, and the H+-ATPase was recovered in a nonactivated form. Trypsin treatment removed the 10-kD C-terminal region from the H+-ATPase as well as the 14-3-3 protein. Using the yeast two-hybrid system, we could show a direct interaction between Arabidopsis 14-3-3 GF14-0 and the last 98 C-terminal amino acids of the Arabidopsis AHA2 plasma membrane H+-ATPase. We propose that the 14-3-3 protein is a natural ligand of the plasma membrane H+-ATPase, regulating proton pumping by displacing the C-terminal autoinhibitory domain of the H+-ATPase.

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