|
THE PLANT CELL, Vol 9, Issue 10 1767-1780, Copyright © 1997 by American Society of Plant Biologists
Induction of Zygotic Polyembryos in Wheat: Influence of Auxin Polar Transport
C. Fischer, V. Speth, S. Fleig-Eberenz and G. Neuhaus
University of Freiburg, Institute of Biology II, Cell Biology, Schanzlestrasse 1, D-79104 Freiburg, Germany
The effects of two auxin polar transport inhibitors, N-1-naphthylphthalamic
acid (NPA) and 3,3[prime],4[prime],5,7-pentahydroxyflavone (quercetin), on
attaining bilateral symmetry from radial symmetry during early wheat
embryogenesis were investigated by using an in vitro culture system.
Although NPA and quercetin belong to two different classes of auxin
transport inhibitors, the phytotropins and the flavonoids, respectively,
they induced the same specific abnormal phenotypes during embryo
development. These abnormal embryos differentiated multiple meristems
(i.e., multiple shoot and root meristems) and multiple organs (i.e.,
multiple coleoptiles and scutella). Multiple shoot apical meristem
phenotypes were characterized by partly multiplied embryonic axes and
supernumerary scutella. The differentiation of multiple primary roots in
addition to multiple shoot meristems and multiple scutella led to the
formation of polyembryos. The occurrence of multiple shoot meristem
phenotypes depended on the concentration of the inhibitor and the
developmental stage of the isolated embryo. Embryos treated with NPA or
quercetin developed multiple radicle phenotypes less frequently than they
developed multiple shoot meristem phenotypes. Our observations suggest that
the root meristem differentiates later than the shoot meristem. Our data
support the hypothesis that polar transport of auxin has a determining
influence on the differentiation of the embryonic axis and the scutellum.
This article has been cited by other articles:
|
|
|
|
 
D. Poli, M. Jacobs, and T. J. Cooke
Auxin regulation of axial growth in bryophyte sporophytes: its potential significance for the evolution of early land plants
Am. J. Botany,
October 1, 2003;
90(10):
1405 - 1415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Rober-Kleber, J. T.P. Albrechtova, S. Fleig, N. Huck, W. Michalke, E. Wagner, V. Speth, G. Neuhaus, and C. Fischer-Iglesias
Plasma Membrane H+-ATPase Is Involved in Auxin-Mediated Cell Elongation during Wheat Embryo Development
Plant Physiology,
March 1, 2003;
131(3):
1302 - 1312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Basu, H. Sun, L. Brian, R. L. Quatrano, and G. K. Muday
Early Embryo Development in Fucus distichus Is Auxin Sensitive
Plant Physiology,
September 1, 2002;
130(1):
292 - 302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Brown, A. M. Rashotte, A. S. Murphy, J. Normanly, B. W. Tague, W. A. Peer, L. Taiz, and G. K. Muday
Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis
Plant Physiology,
June 1, 2001;
126(2):
524 - 535.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Souter and K. Lindsey
Polarity and signalling in plant embryogenesis
J. Exp. Bot.,
June 1, 2000;
51(347):
971 - 983.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T Hamann, U Mayer, and G Jurgens
The auxin-insensitive bodenlos mutation affects primary root formation and apical-basal patterning in the Arabidopsis embryo
Development,
January 4, 1999;
126(7):
1387 - 1395.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K Hadfi, V Speth, and G Neuhaus
Auxin-induced developmental patterns in Brassica juncea embryos
Development,
January 3, 1998;
125(5):
879 - 887.
[Abstract]
[PDF]
|
|
|
|
|
