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THE PLANT CELL, Vol 9, Issue 10 1713-1725, Copyright © 1997 by American Society of Plant Biologists
Promoter Trap Markers Differentiate Structural and Positional Components of Polar Development in Arabidopsis
J. F. Topping and K. Lindsey
Department of Biological Sciences, University of Durham, South Road, Durham DH1 3LE, United Kingdom
To investigate mechanisms involved in establishing polar organization in
Arabidopsis embryos and seedlings, we used promoter trapping to identify
molecular markers ([beta]-glucuronidase fusion genes) expressed in
spatially restricted patterns along the apical-basal axis. Three markers
were identified that are expressed, respectively, in the embryonic and
seedling root tip (POLARIS), cotyledons and shoot and root apices
(EXORDIUM), and root cap (COLUMELLA). Each marker was crossed into the
mutants hydra and emb30, which are defective in embryonic and seedling
morphogenesis. All three markers were expressed in hydra mutants in
patterns similar to those observed in phenotypically wild-type embryos and
seedlings. In emb30 mutants, the EXORDIUM marker was expressed in
cotyledons but not in the expected position of shoot and root meristems,
and the marker COLUMELLA was not expressed at all, which is consistent with
the view that the emb30 mutant, but not hydra, lacks shoot and root
meristems. However, POLARIS was expressed in the basal part of hydra
embryos lacking an embryonic root and in the basal parts of both hydra and
emb30 seedlings. Expression of POLARIS is inducible by exogenous auxin and
suppressed by cytokinin but is unaffected by inhibitors of polar auxin
transport or cell division. We conclude that POLARIS differentiates
positional aspects of polar development from structural aspects.
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