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THE PLANT CELL, Vol 9, Issue 10 1701-1712, Copyright © 1997 by American Society of Plant Biologists
Wounding Changes the Spatial Expression Pattern of the Arabidopsis Plastid [omega]-3 Fatty Acid Desaturase Gene (FAD7) through Different Signal Transduction Pathways
T. Nishiuchi, T. Hamada, H. Kodama and K. Iba
Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-81, Japan
The Arabidopsis FAD7 gene encodes a plastid [omega]-3 fatty acid desaturase
that catalyzes the desaturation of dienoic fatty acids in membrane lipids.
The mRNA levels of the Arabidopsis FAD7 gene in rosette leaves rose rapidly
after local wounding treatments. Wounding also induced the expression of
the FAD7 gene in roots. To study wound-responsive expression of the FAD7
gene in further detail, we analyzed transgenic tobacco plants carrying the
[mdash]825 Arabidopsis FAD7 promoter[mdash][beta]-glucuronidase fusion
gene. In unwounded transformants, FAD7 promoter activity was restricted to
the tissues whose cells contained chloroplasts. Activation of the FAD7
promoter by local wounding treatments was more substantial in stems
(29-fold) and roots (10-fold) of transgenic plants than it was in leaves
(approximately two-fold). Significant induction by wounding was observed in
the overall tissues of stems and included trichomes, the epidermis, cortex,
vascular system, and the pith of the parenchyma. Strong promoter activity
was found preferentially in the vascular tissues of wounded roots. These
results indicate that wounding changes the spatial expression pattern of
the FAD7 gene. Inhibitors of the octadecanoid pathway, salicylic acid and
n-propyl gallate, strongly suppressed the wound activation of the FAD7
promoter in roots but not in leaves or stems. In unwounded plants,
exogenously applied methyl jasmonate activated the FAD7 promoter in roots,
whereas it repressed FAD7 promoter activity in leaves. Taken together,
wound-responsive expression of the FAD7 gene in roots is thought to be
mediated via the octadecanoid pathway, whereas in leaves,
jasmonate-independent wound signals may induce the activation of the FAD7
gene. These observations indicate that wound-responsive expression of the
FAD7 gene in aerial and subterranean parts of plants is brought about by
way of different signal transduction pathways.
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