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THE PLANT CELL, Vol 8, Issue 6 943-958, Copyright © 1996 by American Society of Plant Biologists
S RNase and Interspecific Pollen Rejection in the Genus Nicotiana: Multiple Pollen-Rejection Pathways Contribute to Unilateral Incompatibility between Self-Incompatible and Self-Compatible Species
J. Murfett, T. J. Strabala, D. M. Zurek, B. Mou, B. Beecher and B. A. McClure
University of Missouri-Columbia, Department of Biochemistry, 117 Schweitzer Hall, Columbia, Missouri 65211
In self-incompatible (SI) plants, the S locus acts to prevent growth of
self-pollen and thus promotes outcrossing within the species. Interspecific
crosses between SI and self-compatible (SC) species often show unilateral
incompatibility that follows the SI x SC rule: SI species reject pollen
from SC species, but the reciprocal crosses are usually compatible. The
general validity of the SI x SC rule suggests a link between SI and
interspecific pollen rejection; however, this link has been questioned
because of a number of exceptions to the rule. To clarify the role of the S
locus in interspecific pollen rejection, we transformed several Nicotiana
species and hybrids with genes encoding SA2 or SC10 RNase from SI N. alata.
Compatibility phenotypes in the transgenic plants were tested using pollen
from three SC species showing unilateral incompatibility with N. alata. S
RNase was implicated in rejecting pollen from all three species. Rejection
of N. plumbaginifolia pollen was similar to S allele-specific pollen
rejection, showing a requirement for both S RNase and other genetic factors
from N. alata. In contrast, S RNase-dependent rejection of N. glutinosa and
N. tabacum pollen proceeded without these additional factors. N. alata also
rejects pollen from the latter two species through an S RNase-independent
mechanism. Our results implicate the S locus in all three systems, but it
is clear that multiple mechanisms contribute to interspecific pollen
rejection.
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