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THE PLANT CELL, Vol 8, Issue 4 617-627, Copyright © 1996 by American Society of Plant Biologists
SOS1, a Genetic Locus Essential for Salt Tolerance and Potassium Acquisition
S. J. Wu, L. Ding and J. K. Zhu
Department of Botany and Microbiology, Auburn University, Auburn, Alabama 36849
To begin to determine which genes are essential for salt tolerance in
higher plants, we identified four salt-hypersensitive mutants of
Arabidopsis by using a root-bending assay on NaCl-containing agar plates.
These mutants (sos1-1, sos1-2, sos1-3, and sos1-4) are allelic to each
other and were caused by single recessive nuclear mutations. The SOS1 gene
was mapped to chromosome 2 at 29.5 [plusmn] 6.1 centimorgans. The mutants
showed no phenotypic changes except that their growth was >20 times more
sensitive to inhibition by NaCl. Salt hypersensitivity is a basic cellular
trait exhibited by the mutants at all developmental stages. The sos1
mutants are specifically hypersensitive to Na+ and Li+. The mutants were
unable to grow on media containing low levels (below ~1 mM) of potassium.
Uptake experiments using 86Rb showed that sos1 mutants are defective in
high-affinity potassium uptake. sos1 plants became deficient in potassium
when treated with NaCl. The results demonstrate that potassium acquisition
is a critical process for salt tolerance in glycophytic plants.
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