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THE PLANT CELL, Vol 8, Issue 2 179-188, Copyright © 1996 by American Society of Plant Biologists
Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes
J. J. English, E. Mueller and D. C. Baulcombe
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, United Kingdom
Homology-dependent gene silencing contributes to variation between
transgenic plants with respect to transgene and/or endogenous gene
expression levels. Recent studies have linked post-transcriptional gene
silencing and virus resistance in plants expressing virus-derived
transgenes. Using a potato virus X vector, we present three examples in
which silencing of nonviral transgenes prevented virus accumulation. This
effect was dependent on sequence homology between the virus and the
silenced transgene. Analysis of potato virus X derivatives carrying
bacterial [beta]-glucuronidase (GUS) sequences showed that the 3[prime]
region of the GUS coding sequence was a target of the silencing mechanism
in two independent tobacco lines. Methylation of the silenced GUS
transgenes in these lines was also concentrated in the 3[prime] region of
the GUS coding sequence. Based on this concurrence, we propose a link
between the DNA-based transgene methylation and the RNA-based gene
silencing process.
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