|
THE PLANT CELL, Vol 8, Issue 11 2003-2014, Copyright © 1996 by American Society of Plant Biologists
EF-1[alpha] Is Associated with a Cytoskeletal Network Surrounding Protein Bodies in Maize Endosperm Cells
A. M. Clore, J. M. Dannenhoffer and B. A. Larkins
Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721
By using indirect immunofluorescence and confocal microscopy, we documented
changes in the distribution of elongation factor-1[alpha] (EF-1[alpha]),
actin, and microtubules during the development of maize endosperm cells. In
older interphase cells actively forming starch grains and protein bodies,
the protein bodies are enmeshed in EF-1[alpha] and actin and are found
juxtaposed with a multidirectional array of microtubules. Actin and
EF-1[alpha] appear to exist in a complex, because we observed that the two
are colocalized, and treatment with cytochalasin D resulted in the
redistribution of EF-1[alpa]. These data suggest that EF-1[alpha] and actin
are associated in maize endosperm cells and may help to explain the basis
of the correlation we found between the concentration of EF-1[alpha] and
lysine content. The data also support the hypothesis that the cytoskeleton
plays a role in storage protein deposition. The distributions of
EF-1[alpha] actin, and microtubules change during development. We observed
that in young cells before the accumulation of starch and storage protein,
EF-1[alpha], actin, and microtubules are found mainly in the cell cortex or
in association with nuclei.
This article has been cited by other articles:
|
|
|
|
 
C. Diaz-Camino, R. Conde, N. Ovsenek, and M. A. Villanueva
Actin expression is induced and three isoforms are differentially expressed during germination in Zea mays
J. Exp. Bot.,
February 1, 2005;
56(412):
557 - 565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Crofts, H. Washida, T. W. Okita, M. Ogawa, T. Kumamaru, and H. Satoh
Targeting of Proteins to Endoplasmic Reticulum-Derived Compartments in Plants. The Importance of RNA Localization
Plant Physiology,
November 1, 2004;
136(3):
3414 - 3419.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Lopez-Valenzuela, B. C. Gibbon, D. R. Holding, and B. A. Larkins
Cytoskeletal Proteins Are Coordinately Increased in Maize Genotypes with High Levels of eEF1A
Plant Physiology,
July 1, 2004;
135(3):
1784 - 1797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Lopez-Valenzuela, B. C. Gibbon, P. A. Hughes, T. W. Dreher, and B. A. Larkins
eEF1A Isoforms Change in Abundance and Actin-Binding Activity during Maize Endosperm Development
Plant Physiology,
November 1, 2003;
133(3):
1285 - 1295.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, Y.-m. Woo, C. S. Kim, and B. A. Larkins
Quantitative Trait Locus Mapping of Loci Influencing Elongation Factor 1{alpha} Content in Maize Endosperm
Plant Physiology,
March 1, 2001;
125(3):
1271 - 1282.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Guillen, L. M.L. Lopez-Sanchez, C. S. Roman-Roque, F. Sanchez, and M. A. Villanueva
Biochemical Characterization of Profilin from Seeds of Phaseolus vulgaris L.
Plant Cell Physiol.,
January 1, 2001;
42(1):
54 - 62.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. D. Ransom-Hodgkins, I. Brglez, X. Wang, and W. F. Boss
Calcium-Regulated Proteolysis of eEF1A
Plant Physiology,
March 1, 2000;
122(3):
957 - 966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wang and B. W. Poovaiah
Interaction of Plant Chimeric Calcium/Calmodulin-dependent Protein Kinase with a Homolog of Eukaryotic Elongation Factor-1{alpha}
J. Biol. Chem.,
April 23, 1999;
274(17):
12001 - 12008.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. M. S. Smith and N. V. Raikhel
Nuclear Localization Signal Receptor Importin {alpha} Associates with the Cytoskeleton
PLANT CELL,
November 1, 1998;
10(11):
1791 - 1800.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. D. Ransom, P.-C. Lao, D. A. Gage, and W. F. Boss
Phosphoglycerylethanolamine Posttranslational Modification of Plant Eukaryotic Elongation Factor 1alpha
Plant Physiology,
July 1, 1998;
117(3):
949 - 960.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. C. Gibbon, L. E. Zonia, D. R. Kovar, P. J. Hussey, and C. J. Staiger
Pollen Profilin Function Depends on Interaction with Proline-Rich Motifs
PLANT CELL,
June 1, 1998;
10(6):
981 - 994.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Kranz, P. von Wiegen, H. Quader, and H. Lörz
Endosperm Development after Fusion of Isolated, Single Maize Sperm and Central Cells in Vitro
PLANT CELL,
April 1, 1998;
10(4):
511 - 524.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. K. Morelli, W. Zhou, J. Yu, C. Lu, and M. E. Vayda
Actin Depolymerization Affects Stress-Induced Translational Activity of Potato Tuber Tissue
Plant Physiology,
April 1, 1998;
116(4):
1227 - 1237.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. G. Muench, Y. Wu, S. J. Coughlan, and T. W. Okita
Evidence for a Cytoskeleton-Associated Binding Site Involved in Prolamine mRNA Localization to the Protein Bodies in Rice Endosperm Tissue
Plant Physiology,
February 1, 1998;
116(2):
559 - 569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Kim, Y.-m. Woo, A. M. Clore, R. J. Burnett, N. P. Carneiro, and B. A. Larkins
Zein Protein Interactions, Rather Than the Asymmetric Distribution of Zein mRNAs on Endoplasmic Reticulum Membranes, Influence Protein Body Formation in Maize Endosperm
PLANT CELL,
March 1, 2002;
14(3):
655 - 672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
