|
THE PLANT CELL, Vol 8, Issue 11 1961-1975, Copyright © 1996 by American Society of Plant Biologists
Cell-Specific Expression of Mitochondrial Transcripts in Maize Seedlings
X. Q. Li, M. Zhang and G. G. Brown
Department of Biology, McGill University, 1205 Doctor Penfield Avenue, Montreal, Quebec H3A 1B1, Canada
Although mitochondria are thought to assume crucial and possibly novel
physiological functions during male gametogenesis, it is not known to what
extent mitochondrial function is necessary for other aspects of plant
development or to what degree the expression of plant mitochondrial genes
is subject to cell-specific regulation, particularly during vegetative
growth. We have used in situ hybridization to show that extensive
differences exist in the levels of mitochondrial RNAs (mtRNAs) among
different tissues and among different individual cell types within the same
organ of maize seedlings. The expression of all examined mtRNAs is enhanced
in vascular bundles, particularly in procambium- and xylem-forming cells.
Mitochondrial transcript levels correlated highly with cell division
activity. For example, in roots, the transcripts are abundant in the
dividing cells of the meristem but drop to very low levels in the
nondividing cells of the root cap and the meristem quiescent center. By
comparison, levels of functional mitochondria, as assessed by rhodamine-123
fluorescence, did not vary greatly among the same group of cells. In
shoots, in situ hybridization and blot hybridization revealed differences
in the patterns of localization among different mtRNAs. The results
indicate that during vegetative growth, mitochondrial gene expression at
the transcript level is subject to an unexpected degree of cell-specific
regulation and that different controls may operate on different trancripts.
This article has been cited by other articles:
|
|
|
|
 
A. Elorza, G. Leon, I. Gomez, A. Mouras, L. Holuigue, A. Araya, and X. Jordana
Nuclear SDH2-1 and SDH2-2 Genes, Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II in Arabidopsis, Have Distinct Cell-Specific Expression Patterns and Promoter Activities
Plant Physiology,
December 1, 2004;
136(4):
4072 - 4087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-Q. Li and D. Zhang
Gene Expression Activity and Pathway Selection for Sucrose Metabolism in Developing Storage Root of Sweet Potato
Plant Cell Physiol.,
June 15, 2003;
44(6):
630 - 636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Auger, K. J. Newton, and J. A. Birchler
Nuclear Gene Dosage Effects Upon the Expression of Maize Mitochondrial Genes
Genetics,
April 1, 2001;
157(4):
1711 - 1721.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. F. Ribichich, M. F. Tioni, R. L. Chan, and D. H. Gonzalez
Cell-Type-Specific Expression of Plant Cytochrome c mRNA in Developing Flowers and Roots
Plant Physiology,
April 1, 2001;
125(4):
1603 - 1610.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. A. Felitti, R. L. Chan, M. G. Sierra, and D. H. Gonzalez
The Cytochrome c Gene from the Green Alga Chlamydomonas reinhardtii. Structure and Expression in Wild-Type Cells and in Obligate Photoautotrophic (dk) Mutants
Plant Cell Physiol.,
October 1, 2000;
41(10):
1149 - 1156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Lupold, A. G. F. S. Caoile, and D. B. Stern
Genomic context influences the activity of maize mitochondrial cox2 promoters
PNAS,
September 28, 1999;
96(20):
11670 - 11675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-C. Chang, J. Sheen, M. Bligny, Y. Niwa, S. Lerbs-Mache, and D. B. Stern
Functional Analysis of Two Maize cDNAs Encoding T7-like RNA Polymerases
PLANT CELL,
May 1, 1999;
11(5):
911 - 926.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Mackenzie and L. McIntosh
Higher Plant Mitochondria
PLANT CELL,
April 1, 1999;
11(4):
571 - 586.
[Full Text]
|
|
|
|
|
|
|
D. S. Lupold, A. G. F. S. Caoile, and D. B. Stern
The Maize Mitochondrial cox2 Gene Has Five Promoters in Two Genomic Regions, Including a Complex Promoter Consisting of Seven Overlapping Units
J. Biol. Chem.,
February 5, 1999;
274(6):
3897 - 3903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-Q. Li, M. Jean, B. S. Landry, and G. G. Brown
Restorer genes for different forms of Brassica cytoplasmic male sterility map to a single nuclear locus that modifies transcripts of several mitochondrial genes
PNAS,
August 18, 1998;
95(17):
10032 - 10037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sarria, A. Lyznik, C. E. Vallejos, and S. A. Mackenzie
A Cytoplasmic Male Sterility–Associated Mitochondrial Peptide in Common Bean Is Post-Translationally Regulated
PLANT CELL,
July 1, 1998;
10(7):
1217 - 1228.
[Abstract]
[Full Text]
|
|
|
|
|
