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THE PLANT CELL, Vol 6, Issue 6 835-843, Copyright © 1994 by American Society of Plant Biologists
Identification and Characterization of cDNA Clones Encoding Plant Calreticulin in Barley
F. Chen, P. M. Hayes, D. M. Mulrooney and A. Pan
Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon 97331-3002
Two cDNA clones (CRH1 and CRH2) homologous to animal calreticulin, a major
calcium storage protein in the lumen of the endoplasmic reticulum, were
isolated from an ovary cDNA library of barley through differential
screening. The two clones differ in the 3[prime] untranslated region and
the 5[prime] region that encodes a putative N-terminal signal sequence.
CRH1 was mapped to the minus arm of chromosome 1. CRH2 was mapped to the
minus arm of chromosome 2. The deduced amino acid sequences share 50 to 55%
identity with animal calreticulins and exhibit the same three-zone
characteristic. Recombinant protein stained blue with Stains-all and bound
45Ca2+ when transferred to nitrocellulose membranes. A native protein of
~55 kD was identified in ovary extract. Elevated gene expression was
observed in ovaries 1 day after pollination and during early embryogenesis.
CRH1 was expressed at a higher level than CRH2. These studies demonstrate
the presence of calreticulin in plant cells and its developmental
regulation in fertilization.
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