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THE PLANT CELL, Vol 6, Issue 6 799-810, Copyright © 1994 by American Society of Plant Biologists
Promoter Elements Required for Developmental Expression of the Maize Adh1 Gene in Transgenic Rice
J. Kyozuka, M. Olive, W. J. Peacock, E. S. Dennis and K. Shimamoto
Plantech Research Institute, 1000 Kamoshida, Midori-ku, Yokohama 227, Japan
To define the regions of the maize alcohol dehydrogenase 1 (Adh1) promoter
that confer tissue-specific expression, a series of 5[prime] promoter
deletions and substitution mutations were linked to the Escherichia coli
[beta]-glucuronidase A (uidA) reporter gene and introduced into rice
plants. A region between -140 and -99 not only conferred anaerobically
inducible expression in the roots of transgenic plants but was also
required for expression in the root cap, embryo, and in endosperm under
aerobic conditions. GC-rich (GC-1, GC-2, and GC-3) or GT-rich (GT-1 and
GT-2) sequence motifs in this region were necessary for expression in these
tissues, as they were in anaerobic expression. Expression in the root cap
under aerobic conditions required all the GC- and GT-rich motifs. The GT-1,
GC-1, GC-2, and GC-3 motifs, and to a lesser extent the GT-2 motif, were
also required for anaerobic responsiveness in rice roots. All elements
except the GC-3 motif were needed for endosperm-specific expression. The
GC-2 motif and perhaps the GT-1 motif appeared to be the only elements
required for high-level expression in the embryos of rice seeds. Promoter
regions important for shoot-, embryo-, and pollen-specific expression were
proximal to -99, and nucleotides required for shoot-specific expression
occurred between positions -72 and -43. Pollen-specific expression required
a sequence element outside the promoter region, between +54 and +106 of the
untranslated leader, as well as a silencer element in the promoter between
-72 and -43.
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