Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (42)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yunes, J. A.
Right arrow Articles by Arruda, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yunes, J. A.
Right arrow Articles by Arruda, P.
Agricola
Right arrow Articles by Yunes, J. A.
Right arrow Articles by Arruda, P.

THE PLANT CELL, Vol 6, Issue 2 237-249, Copyright © 1994 by American Society of Plant Biologists

RESEARCH ARTICLES

The Transcriptional Activator Opaque2 Recognizes Two Different Target Sequences in the 22-kD-like [alpha]-Prolamin Genes

J. A. Yunes, G. C. Neto, M. J. da Silva, A. Leite, LMM. Ottoboni and P. Arruda
Centro de Biologia Molecular e Engenharia Genetica, Universidade Estadual de Campinas, 13081, Campinas, SP, Brazil

The maize Opaque2 (O2) protein is a "leucine zipper" DNA binding factor that interacts with the sequence TCCACGTAGA in the promoters of the 22-kD [alpha]-zein genes and activates its transcription. A completely different consensus sequence (GATGAPyPuTGPu) identified in b-32, a gene that encodes an abundant albumin that is also under control of the O2 locus, can also be bound by the O2 protein. We showed that the gene encoding the 22-kD-like [alpha]-coixin, the [alpha]-prolamin of the maize-related grass Coix, can also be transactivated by the O2 protein. A binding assay in vitro and footprint analysis demonstrated that the GACATGTC sequence of the [alpha]-coixin promoter can be recognized and protected by the maize O2 protein. Employing transient expression experiments in immature maize endosperm and tobacco mesophyll protoplasts, we demonstrated that the O2 protein can activate expression of the [beta]-glucuronidase reporter gene placed under the control of the 22-kD-like [alpha]-coixin promoter. We also demonstrated that a 22-kD-like [alpha]-coixin pseudogene promoter is transactivated by the maize O2 protein.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
F. Gavazzi, B. Lazzari, P. Ciceri, E. Gianazza, and A. Viotti
Wild-Type Opaque2 and Defective opaque2 Polypeptides Form Complexes in Maize Endosperm Cells and Bind the Opaque2-Zein Target Site
Plant Physiology, November 1, 2007; 145(3): 933 - 945.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Song, G. Segal, and J. Messing
Expression of the sorghum 10-member kafirin gene cluster in maize endosperm
Nucleic Acids Res., December 29, 2004; 32(22): e189 - e189.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Lara, L. Onate-Sanchez, Z. Abraham, C. Ferrandiz, I. Diaz, P. Carbonero, and J. Vicente-Carbajosa
Synergistic Activation of Seed Storage Protein Gene Expression in Arabidopsis by ABI3 and Two bZIPs Related to OPAQUE2
J. Biol. Chem., May 30, 2003; 278(23): 21003 - 21011.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Y. Kim, J. Ma, P. Perret, Z. Li, and T. L. Thomas
Arabidopsis ABI5 Subfamily Members Have Distinct DNA-Binding and Transcriptional Activities
Plant Physiology, October 1, 2002; 130(2): 688 - 697.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Yang, L. Wu, Y.-S. Hwang, L. Chen, and N. Huang
Expression of the REB transcriptional activator in rice grains improves the yield of recombinant proteins whose genes are controlled by a Reb-responsive promoter
PNAS, September 25, 2001; 98(20): 11438 - 11443.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Ciceri, S. Castelli, M. Lauria, B. Lazzari, A. Genga, L. Bernard, M. Sturaro, and A. Viotti
Specific Combinations of Zein Genes and Genetic Backgrounds Influence the Transcription of the Heavy-Chain Zein Genes in Maize opaque-2 Endosperms
Plant Physiology, September 1, 2000; 124(1): 451 - 460.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
L. Onate, J. Vicente-Carbajosa, P. Lara, I. Diaz, and P. Carbonero
Barley BLZ2, a Seed-specific bZIP Protein That Interacts with BLZ1 in Vivo and Activates Transcription from the GCN4-like motif of B-hordein Promoters in Barley Endosperm
J. Biol. Chem., April 2, 1999; 274(14): 9175 - 9182.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. A. Yunes, A. L. Vettore, M. J. da Silva, A. Leite, and P. Arruda
Cooperative DNA Binding and Sequence Discrimination by the Opaque2 bZIP Factor
PLANT CELL, November 1, 1998; 10(11): 1941 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Rossi, M. Motto, and L. Pellegrini
Analysis of the Methylation Pattern of the Maize Opaque-2 (O2) Promoter and in Vitro Binding Studies Indicate That the O2 B-Zip Protein and Other Endosperm Factors Can Bind to Methylated Target Sequences
J. Biol. Chem., May 23, 1997; 272(21): 13758 - 13765.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1994 by the American Society of Plant Biologists