Plant Cell Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (198)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pierson, E. S.
Right arrow Articles by Hepler, P. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pierson, E. S.
Right arrow Articles by Hepler, P. K.
Agricola
Right arrow Articles by Pierson, E. S.
Right arrow Articles by Hepler, P. K.

THE PLANT CELL, Vol 6, Issue 12 1815-1828, Copyright © 1994 by American Society of Plant Biologists

RESEARCH ARTICLES

Pollen Tube Growth Is Coupled to the Extracellular Calcium Ion Flux and the Intracellular Calcium Gradient: Effect of BAPTA-Type Buffers and Hypertonic Media

E. S. Pierson, D. D. Miller, D. A. Callaham, A. M. Shipley, B. A. Rivers, M. Cresti and P. K. Hepler
Department of Biology, Morrill Science Center III, University of Massachusetts, Amherst, Massachusetts 01003

Lily pollen tubes possess a steep, tip-focused intracellular Ca2+ gradient and a tip-directed extracellular Ca2+ influx. Ratiometric ion imaging revealed that the gradient extends from above 3.0 [mu]M at the apex to ~0.2 [mu]M within 20 [mu]m from the tip, while application of the Ca2+-specific vibrating electrode indicated that the extracellular influx measured between 1.4 and 14 pmol cm-2 sec-1. We examined the relationship between these phenomena and their role in tube growth by using different 1,2-bis(o-aminophenoxy)ethane N,N,N[prime],N[prime]-tetraacetic acid (BAPTA)-type buffers and hypertonic media. Injection of active BAPTA-type buffers or application of elevated levels of sucrose reversibly inhibited growth, destroyed tip zonation of organelles, and modified normal patterns of cytoplasmic streaming. Simultaneously, these treatments dissipated both the intracellular tip-focused gradient and the extracellular Ca2+ flux. Of the BAPTA-type buffers, 5,5[prime]-dibromo-BAPTA (dissociation constant [Kd] is 1.5 [mu]M) and 4,4[prime]-difluoro-BAPTA (Kd of 1.7 [mu]M) exhibited greater activity than those buffers with either a higher affinity (5,5[prime]-dimethyl-BAPTA, Kd of 0.15 [mu]M; BAPTA, Kd of 0.21 [mu]M; 5,5[prime]- difluoro-BAPTA, Kd of 0.25 [mu]M) or lower affinity (5-methyl, 5[prime]-nitro-BAPTA, Kd of 22 [mu]M) for Ca2+. Our findings provide evidence that growing pollen tubes have open Ca2+ channels in their tip and that these channels become inactivated in nongrowing tubes. The studies with elevated sucrose support the view that stretching of the apical plasma membrane contributes to the maintenance of the Ca2+ signal.


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., December 26, 2008; (2008) ern321v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
A. M. Prado, R. Colaco, N. Moreno, A. C. Silva, and J. A. Feijo
Targeting of Pollen Tubes to Ovules Is Dependent on Nitric Oxide (NO) Signaling
Mol Plant, July 1, 2008; 1(4): 703 - 714.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
L. L. Ge, H. Q. Tian, and S. D. Russell
Calcium function and distribution during fertilization in angiosperms
Am. J. Botany, June 1, 2007; 94(6): 1046 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. M. Yoon, P. E. Dowd, S. Gilroy, and A. G. McCubbin
Calcium-Dependent Protein Kinase Isoforms in Petunia Have Distinct Functions in Pollen Tube Growth, Including Regulating Polarity
PLANT CELL, April 1, 2006; 18(4): 867 - 878.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Z. Xu and H. K. Dooner
The Maize aberrant pollen transmission 1 Gene Is a SABRE/KIP Homolog Required for Pollen Tube Growth
Genetics, February 1, 2006; 172(2): 1251 - 1261.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J.-U. Hwang, Y. Gu, Y.-J. Lee, and Z. Yang
Oscillatory ROP GTPase Activation Leads the Oscillatory Polarized Growth of Pollen Tubes
Mol. Biol. Cell, November 1, 2005; 16(11): 5385 - 5399.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. D. Lazzaro, L. Cardenas, A. P. Bhatt, C. D. Justus, M. S. Phillips, T. L. Holdaway-Clarke, and P. K. Hepler
Calcium gradients in conifer pollen tubes; dynamic properties differ from those seen in angiosperms
J. Exp. Bot., October 1, 2005; 56(420): 2619 - 2628.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Yokota, M. Tominaga, I. Mabuchi, Y. Tsuji, C. J. Staiger, K. Oiwa, and T. Shimmen
Plant Villin, Lily P-135-ABP, Possesses G-Actin Binding Activity and Accelerates the Polymerization and Depolymerization of Actin in a Ca2+-Sensitive Manner
Plant Cell Physiol., October 1, 2005; 46(10): 1690 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Bosch, A. Y. Cheung, and P. K. Hepler
Pectin Methylesterase, a Regulator of Pollen Tube Growth
Plant Physiology, July 1, 2005; 138(3): 1334 - 1346.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. Gu, Y. Fu, P. Dowd, S. Li, V. Vernoud, S. Gilroy, and Z. Yang
A Rho family GTPase controls actin dynamics and tip growth via two counteracting downstream pathways in pollen tubes
J. Cell Biol., April 11, 2005; 169(1): 127 - 138.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Z.-l. Shang, L.-g. Ma, H.-l. Zhang, R.-r. He, X.-c. Wang, S.-j. Cui, and D.-y. Sun
Ca2+ Influx into Lily Pollen Grains Through a Hyperpolarization-activated Ca2+-permeable Channel Which Can be Regulated by Extracellular CaM
Plant Cell Physiol., April 1, 2005; 46(4): 598 - 608.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. S. Tegg, L. Melian, C. R. Wilson, and S. Shabala
Plant Cell Growth and Ion Flux Responses to the Streptomycete Phytotoxin Thaxtomin A: Calcium and Hydrogen Flux Patterns Revealed by the Non-invasive MIFE Technique
Plant Cell Physiol., April 1, 2005; 46(4): 638 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Xu, C. A. Brearley, W.-H. Lin, Y. Wang, R. Ye, B. Mueller-Roeber, Z.-H. Xu, and H.-W. Xue
A Role of Arabidopsis Inositol Polyphosphate Kinase, AtIPK2{alpha}, in Pollen Germination and Root Growth
Plant Physiology, January 1, 2005; 137(1): 94 - 103.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-F. Wang, L.-M. Fan, W.-Z. Zhang, W. Zhang, and W.-H. Wu
Ca2+-Permeable Channels in the Plasma Membrane of Arabidopsis Pollen Are Regulated by Actin Microfilaments
Plant Physiology, December 1, 2004; 136(4): 3892 - 3904.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Iwano, H. Shiba, T. Miwa, F.-S. Che, S. Takayama, T. Nagai, A. Miyawaki, and A. Isogai
Ca2+ Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis
Plant Physiology, November 1, 2004; 136(3): 3562 - 3571.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Schiott, S. M. Romanowsky, L. Baekgaard, M. K. Jakobsen, M. G. Palmgren, and J. F. Harper
A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization
PNAS, June 22, 2004; 101(25): 9502 - 9507.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Yadegari and G. N. Drews
Female Gametophyte Development
PLANT CELL, June 1, 2004; 16(suppl_1): S133 - S141.
[Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
L. M. STONE, K. A. SEATON, J. KUO, and J. A. McCOMB
Fast Pollen Tube Growth in Conospermum Species
Ann. Bot., April 1, 2004; 93(4): 369 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Iwano, T. Entani, H. Shiba, S. Takayama, and A. Isogai
Calcium Crystals in the Anther of Petunia: the Existence and Biological Significance in the Pollination Process
Plant Cell Physiol., January 15, 2004; 45(1): 40 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Golovkin and A. S. N. Reddy
A calmodulin-binding protein from Arabidopsis has an essential role in pollen germination
PNAS, September 2, 2003; 100(18): 10558 - 10563.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
L. B. Silverman-Gavrila and R. R. Lew
Calcium gradient dependence of Neurospora crassa hyphal growth
Microbiology, September 1, 2003; 149(9): 2475 - 2485.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Ojha and F. Barja
Spatial and cellular localization of calcium-dependent protease (CDP II) in Allomyces arbuscula
J. Cell Sci., March 15, 2003; 116(6): 1095 - 1105.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Zonia and J. A. Feijo
State and Spectral Properties of Chloride Oscillations in Pollen
Biophys. J., February 1, 2003; 84(2): 1387 - 1398.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Camacho and R. Malho
Endo/exocytosis in the pollen tube apex is differentially regulated by Ca2+ and GTPases
J. Exp. Bot., January 1, 2003; 54(380): 83 - 92.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Gu, V. Vernoud, Y. Fu, and Z. Yang
ROP GTPase regulation of pollen tube growth through the dynamics of tip-localized F-actin
J. Exp. Bot., January 1, 2003; 54(380): 93 - 101.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. J. Staiger and V. E. Franklin-Tong
The actin cytoskeleton is a target of the self-incompatibility response in Papaver rhoeas
J. Exp. Bot., January 1, 2003; 54(380): 103 - 113.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
K. R. Robinson and M. A. Messerli
Pulsating Ion Fluxes and Growth at the Pollen Tube Tip
Sci. Signal., December 10, 2002; 2002(162): pe51 - pe51.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Zonia, S. Cordeiro, J. Tupy, and J. A. Feijo
Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate
PLANT CELL, September 1, 2002; 14(9): 2233 - 2249.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Inoue, S.-y. Yamada, Y. Nagata, and T. Shimmen
Rhizoid Differentiation in Spirogyra: Position Sensing by Terminal Cells
Plant Cell Physiol., May 15, 2002; 43(5): 479 - 483.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Kachroo, M. E. Nasrallah, and J. B. Nasrallah
Self-Incompatibility in the Brassicaceae: Receptor-Ligand Signaling and Cell-to-Cell Communication
PLANT CELL, May 1, 2002; 14(90001): S227 - 238.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Mouline, A.-A. Very, F. Gaymard, J. Boucherez, G. Pilot, M. Devic, D. Bouchez, J.-B. Thibaud, and H. Sentenac
Pollen tube development and competitive ability are impaired by disruption of a Shaker K+ channel in Arabidopsis
Genes & Dev., February 1, 2002; 16(3): 339 - 350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. N. Reddy, I. S. Day, S. B. Narasimhulu, F. Safadi, V. S. Reddy, M. Golovkin, and M. J. Harnly
Isolation and Characterization of a Novel Calmodulin-binding Protein from Potato
J. Biol. Chem., February 1, 2002; 277(6): 4206 - 4214.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. V. Minorsky

Plant Physiology, September 1, 2001; 127(1): 8 - 9.
[Full Text] [PDF]

Home page
 J Exp BotHome page
L.-M. Fan, Y.-F. Wang, H. Wang, and W.-H. Wu
In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts
J. Exp. Bot., August 1, 2001; 52(361): 1603 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Vidali, S. T. McKenna, and P. K. Hepler
Actin Polymerization Is Essential for Pollen Tube Growth
Mol. Biol. Cell, August 1, 2001; 12(8): 2534 - 2545.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. F. Antoine, J.-E. Faure, S. Cordeiro, C. Dumas, M. Rougier, and J. A. Feijó
A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants
PNAS, September 5, 2000; (2000) 180243697.
[Abstract] [Full Text]

Home page
 Plant CellHome page
G. Cai, S. Romagnoli, A. Moscatelli, E. Ovidi, G. Gambellini, A. Tiezzi, and M. Cresti
Identification and Characterization of a Novel Microtubule-Based Motor Associated with Membranous Organelles in Tobacco Pollen Tubes
PLANT CELL, September 1, 2000; 12(9): 1719 - 1736.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. Geitmann, B. N. Snowman, A. M. C. Emons, and V. E. Franklin-Tong
Alterations in the Actin Cytoskeleton of Pollen Tubes Are Induced by the Self-Incompatibility Reaction in Papaver rhoeas
PLANT CELL, July 1, 2000; 12(7): 1239 - 1252.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
E. Yokota, S. Muto, and T. Shimmen
Calcium-Calmodulin Suppresses the Filamentous Actin-Binding Activity of a 135-Kilodalton Actin-Bundling Protein Isolated from Lily Pollen Tubes
Plant Physiology, June 1, 2000; 123(2): 645 - 654.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
W. D. Ransom-Hodgkins, I. Brglez, X. Wang, and W. F. Boss
Calcium-Regulated Proteolysis of eEF1A
Plant Physiology, March 1, 2000; 122(3): 957 - 966.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Li, Y. Lin, R. M. Heath, M. X. Zhu, and Z. Yang
Control of Pollen Tube Tip Growth by a Rop GTPase –Dependent Pathway That Leads to Tip-Localized Calcium Influx
PLANT CELL, September 1, 1999; 11(9): 1731 - 1742.
[Abstract] [Full Text]

Home page
 Plant CellHome page
V. E. Franklin-Tong
Signaling and the Modulation of Pollen Tube Growth
PLANT CELL, April 1, 1999; 11(4): 727 - 738.
[Full Text]

Home page
 JCBHome page
J.A. Feijo, J. Sainhas, G.R. Hackett, J.G. Kunkel, and P.K. Hepler
Growing Pollen Tubes Possess a Constitutive Alkaline Band in the Clear Zone and a Growth-dependent Acidic Tip
J. Cell Biol., February 8, 1999; 144(3): 483 - 496.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Messerli, G Danuser, and K. Robinson
Pulsatile influxes of H+, K+ and Ca2+ lag growth pulses of Lilium longiflorum pollen tubes
J. Cell Sci., January 5, 1999; 112(10): 1497 - 1509.
[Abstract] [PDF]

Home page
 Plant Physiol.Home page
E. Yokota, S. Muto, and T. Shimmen
Inhibitory Regulation of Higher-Plant Myosin by Ca2+ Ions
Plant Physiology, January 1, 1999; 119(1): 231 - 240.
[Abstract] [Full Text]

Home page
 Plant CellHome page
J. Muschietti, Y. Eyal, and S. McCormick
Pollen Tube Localization Implies a Role in Pollen–Pistil Interactions for the Tomato Receptor-like Protein Kinases LePRK1 and LePRK2
PLANT CELL, March 1, 1998; 10(3): 319 - 330.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. G. W. Kaminskyj and J. E. Hamer
hyp Loci Control Cell Pattern Formation in the Vegetative Mycelium of Aspergillus nidulans
Genetics, February 1, 1998; 148(2): 669 - 680.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. L. Jones, L. V. Kochian, and S. Gilroy
Aluminum Induces a Decrease in Cytosolic Calcium Concentration in BY-2 Tobacco Cell Cultures
Plant Physiology, January 1, 1998; 116(1): 81 - 89.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
M. Fricker, N. White, and G Obermeyer
pH gradients are not associated with tip growth in pollen tubes of Lilium longiflorum
J. Cell Sci., January 8, 1997; 110(15): 1729 - 1740.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
M Messerli and K. Robinson
Tip localized Ca2+ pulses are coincident with peak pulsatile growth rates in pollen tubes of Lilium longiflorum
J. Cell Sci., January 6, 1997; 110(11): 1269 - 1278.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
R. Parton, S Fischer, R Malho, O Papasouliotis, T. Jelitto, T Leonard, and N. Read
Pronounced cytoplasmic pH gradients are not required for tip growth in plant and fungal cells
J. Cell Sci., January 5, 1997; 110(10): 1187 - 1198.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
K. S. Schumaker and M. J. Gizinski
1,4-Dihydropyridine Binding Sites in Moss Plasma Membranes
J. Biol. Chem., October 6, 1995; 270(40): 23461 - 23467.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. Johnson, M. Knight, T Kondo, P Masson, J Sedbrook, A Haley, and A Trewavas
Circadian oscillations of cytosolic and chloroplastic free calcium in plants
Science, September 29, 1995; 269(5232): 1863 - 1865.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
N. Levina, R. Lew, G. Hyde, and I. Heath
The roles of Ca2+ and plasma membrane ion channels in hyphal tip growth of Neurospora crassa
J. Cell Sci., January 11, 1995; 108(11): 3405 - 3417.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
F. Safadi, V. S. Reddy, and A. S. N. Reddy
A Pollen-specific Novel Calmodulin-binding Protein with Tetratricopeptide Repeats
J. Biol. Chem., November 3, 2000; 275(45): 35457 - 35470.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. F. Antoine, J.-E. Faure, S. Cordeiro, C. Dumas, M. Rougier, and J. A. Feijo
A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants
PNAS, September 12, 2000; 97(19): 10643 - 10648.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1994 by the American Society of Plant Biologists