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THE PLANT CELL, Vol 5, Issue 6 603-613, Copyright © 1993 by American Society of Plant Biologists
Scaffold Attachment Regions Increase Reporter Gene Expression in Stably Transformed Plant Cells
G. C. Allen, G. E. Hall Jr, L. C. Childs, A. K. Weissinger, S. Spiker and W. F. Thompson
Department of Botany, North Carolina State University, Raleigh, North Carolina 27695
The yeast ARS-1 element contains a scaffold attachment region (SAR) that we
have previously shown can bind to plant nuclear scaffolds in vitro. To test
effects on expression, constructs in which a chimeric [beta]-glucuronidase
(GUS) gene was flanked by this element were delivered into tobacco
suspension cells by microprojectile bombardment. In stably transformed cell
lines, GUS activity averaged 12-fold higher (24-fold on a gene copy basis)
for a construct containing two flanking SARs than for a control construct
lacking SARs. Expression levels were not proportional to gene copy number,
as would have been predicted if the element simply reduced position effect
variation. Instead, the element appeared to reduce an inhibitory effect on
expression in certain transformants containing multiple gene copies. The
effect on expression appears to require chromosomal integration, because
SAR constructs were only twofold more active than the controls in transient
assays.
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