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THE PLANT CELL, Vol 5, Issue 4 371-378, Copyright © 1993 by American Society of Plant Biologists
Tagging and Cloning of a Petunia Flower Color Gene with the Maize Transposable Element Activator
G. Chuck, T. Robbins, C. Nijjar, E. Ralston, N. Courtney-Gutterson and H. K. Dooner
DNA Plant Technology Corporation, 6701 San Pablo Avenue, Oakland, California 94608
We report here the use of the maize transposable element Activator (Ac) to
isolate a dicot gene. Ac was introduced into petunia, where it transposed
into Ph6, one of several genes that modify anthocyanin pigmentation in
flowers by affecting the pH of the corolla. Like other Ac-mutable alleles,
the new mutation is unstable and reverts to a functional form in somatic
and germinal tissues. The mutant gene was cloned using Ac as a probe,
demonstrating the feasibility of heterologous transposon tagging in higher
plants. Confirmation that the cloned DNA fragment corresponded to the
mutated gene was obtained from an analysis of revertants. In every case
examined, reversion to the wild-type phenotype was correlated with
restoration of a wild-type-sized DNA fragment. New transposed Acs were
detected in many of the revertants. As in maize, the frequency of somatic
and germinal excision of Ac from the mutable allele appears to be dependent
on genetic background.
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