THE PLANT CELL, Vol 5, Issue 3 277-288, Copyright © 1993 by American Society of Plant Biologists
Developmental Expression and Localization of Petunia Glycine-Rich Protein 1
C. M. Condit
Department of Biochemistry/Mail Stop 200, University of Nevada, Reno, Nevada 89557-0014
An anti-petunia glycine-rich protein 1 (ptGRP1) antibody was used for
biotin-streptavidin-alkaline phosphatase localization of this protein. In
petunia stem and leaves grown under different light conditions, these
studies revealed a complex pattern of cell localization for this protein.
Levels of ptGRP1 were shown to decrease with developmental age of the
tissue, appearing to correlate directly with expansive growth and inversely
with lignification. Significantly, plants grown under low light (~32
[mu]mol m-2 sec-1 at noon) showed at least an eightfold increased level of
ptGRP1 protein throughout ptGRP1's expression period when compared to
plants grown under higher light (~80 [mu]mol m-2 sec-1 at noon). Evidence
also indicated that for one cell type in which ptGRP1 is localized, this
protein is imported rather than synthesized. In addition, confocal
microscopy studies suggested that ptGRP1 is deposited at the cell
wall/membrane interface rather than within the cell wall.