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THE PLANT CELL, Vol 5, Issue 3 241-251, Copyright © 1993 by American Society of Plant Biologists
Identification of a Methyl Jasmonate-Responsive Domain in the Soybean vspB Promoter
H. S. Mason, D. B. DeWald and J. E. Mullet
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128
Soybean vspB encodes a highly expressed vegetative storage protein-acid
phosphatase. In soybean, vspB expression is stimulated by methyl jasmonate
(MeJA) and sugars. The vspB promoter was studied by transforming tobacco
with fusions of 5[prime] noncoding vspB DNA and the gene encoding
[beta]-glucuronidase (GUS). Constructs containing 833 bp of vspB 5[prime]
DNA showed high expression of GUS in stems, leaf veins and trichomes,
sepals, and pollen. Sucrose (0.2 M) and MeJA (10-5 M) increased gene
expression when applied to leaf tissue. Deletion of the region -787 to -520
with respect to the transcription initiation site rendered the vspB
promoter noninducible by MeJA but still sucrose responsive. This result
indicates that DNA elements capable of modulating vspB by MeJA can be
separated from carbon response elements. Further 5[prime] end deletion from
-520 to -403 or 3[prime] end deletion from -165 to -289 removed DNA
sequences involved in carbon modulation of gene expression. A DNA domain
that mediates the MeJA response was further localized to a 50-bp region
between -535 and -585. This domain when fused to a cauliflower mosaic virus
(CaMV) 35S truncated (-88) promoter makes the CaMV promoter responsive to
MeJA. The MeJA-responsive domain contains a G-box motif (CACGTG) and a
C-rich sequence. A similar 50-bp DNA region is present in the putative
promoter of vspA. Related sequences are located in a wound- and
MeJA-responsive domain of the proteinase inhibitor II gene and a
UV-responsive promoter domain of chs, the gene encoding chalcone synthase
that is also responsive to MeJA.
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