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THE PLANT CELL, Vol 5, Issue 12 1761-1769, Copyright © 1993 by American Society of Plant Biologists
rha1, a Gene Encoding a Small GTP Binding Protein from Arabidopsis, Is Expressed Primarily in Developing Guard Cells
N. Terryn, M. B. Arias, G. Engler, C. Tire, R. Villarroel, M. Van Montagu and D. Inze
Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
The rha1 gene from Arabidopsis encodes a small GTP binding protein
belonging to the Ypt/Rab family. Transgenic Arabidopsis plants containing
the promoter region of the rha1 gene fused to the [beta]-glucuronidase
(gus) reporter gene revealed gus expression limited mainly to the guard
cells of stomata, the stipules, and the root tip of young plants. In
flowering plants, expression was found predominantly in the receptacle and
in guard cells of the different flower organs. High GUS activity could also
be seen in callus tissue and developing seeds. No detectable activity was
present in other plant tissues; activity could not be induced by various
treatments. GUS activity was visualized histochemically using both
5-bromo-4-chloro-3-indolyl [beta]-D-glucuronide and a newly developed GUS
substrate: Sudan II-[beta]-glucuronide. The latter precipitates as red
crystals at the site of GUS activity. Results obtained by the gus analysis
were confirmed by whole-mount mRNA in situ hybridization. A hypothesis for
the function of the Rha1 protein is discussed.
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