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THE PLANT CELL, Vol 3, Issue 2 203-212, Copyright © 1991 by American Society of Plant Biologists
Biosynthesis of the Chloroplast Cytochrome b6f Complex: Studies in a Photosynthetic Mutant of Lemna
B. D. Bruce and R. Malkin
Department of Plant Biology, University of California, Berkeley, Berkeley, California 94720
The biosynthesis of the cytochrome b6f complex has been studied in a
mutant, no. 1073, of Lemna perpusilla that contained less than 1% of the
four protein subunits when compared with a wild-type strain. RNA gel blot
analyses of the mutant indicated that the chloroplast genes for cytochrome
f, cytochrome b6, and subunit IV (petA, petB, and petD, respectively) are
transcribed and that the petB and petD transcripts undergo their normal
processing. Analysis of polysomal polyA+ RNA indicated that the level of
translationally active mRNA for the nuclear-encoded Rieske Fe-S protein
(petC) was reduced by >100-fold in the mutant. Immunoprecipitation of in
vivo labeled proteins indicated that both cytochrome f and subunit IV are
synthesized and that subunit IV has a 10-fold higher rate of protein
turnover in the mutant. These results are discussed in terms of the
assembly of the cytochrome complex and the key role of the Rieske Fe-S
protein in this process.
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