OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 19/8/2470    most recent tpc.107.050708v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Yan, Y. Articles by Farmer, E. E. Search for Related Content PubMed PubMed Citation Articles by Yan, Y. Articles by Farmer, E. E. Agricola Articles by Yan, Y. Articles by Farmer, E. E.

A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway^[W],[OA]

^a Department of Plant Molecular Biology, University of Lausanne, Biophore, CH-1015 Lausanne, Switzerland
^b Swiss Institute of Bioinformatics, Vital-IT Group, Génopode, CH-1015 Lausanne, Switzerland

² Address correspondence to edward.farmer{at}unil.ch.

Wounding plant tissues initiates large-scale changes in transcription coupled to growth arrest, allowing resource diversion for defense. These processes are mediated in large part by the potent lipid regulator jasmonic acid (JA). Genes selected from a list of wound-inducible transcripts regulated by the jasmonate pathway were overexpressed in Arabidopsis thaliana, and the transgenic plants were then assayed for sensitivity to methyl jasmonate (MeJA). When grown in the presence of MeJA, the roots of plants overexpressing a gene of unknown function were longer than those of wild-type plants. When transcript levels for this gene, which we named JASMONATE-ASSOCIATED1 (JAS1), were reduced by RNA interference, the plants showed increased sensitivity to MeJA and growth was inhibited. These gain- and loss-of-function assays suggest that this gene acts as a repressor of JA-inhibited growth. An alternative transcript from the gene encoding a second protein isoform with a longer C terminus failed to repress jasmonate sensitivity. This identified a conserved C-terminal sequence in JAS1 and related genes, all of which also contain Zim motifs and many of which are jasmonate-regulated. Both forms of JAS1 were found to localize to the nucleus in transient expression assays. Physiological tests of growth responses after wounding were consistent with the fact that JAS1 is a repressor of JA-regulated growth retardation.

This article has been cited by other articles:

				A. K. Spartz and W. M. Gray Plant hormone receptors: new perceptions Genes & Dev., August 15, 2008; 22(16): 2139 - 2148. [Abstract] [Full Text] [PDF]

				P. D. Nabity, J. A. Zavala, and E. H. Delucia Indirect Suppression of Photosynthesis on Individual Leaves by Arthropod Herbivory Ann. Bot., July 26, 2008; (2008) mcn127v1. [Abstract] [Full Text] [PDF]

				C. Tretner, U. Huth, and B. Hause Mechanostimulation of Medicago truncatula leads to enhanced levels of jasmonic acid J. Exp. Bot., July 1, 2008; 59(10): 2847 - 2856. [Abstract] [Full Text] [PDF]

				T. Shoji, T. Ogawa, and T. Hashimoto Jasmonate-Induced Nicotine Formation in Tobacco is Mediated by Tobacco COI1 and JAZ Genes Plant Cell Physiol., July 1, 2008; 49(7): 1003 - 1012. [Abstract] [Full Text] [PDF]

				G. Glauser, E. Grata, L. Dubugnon, S. Rudaz, E. E. Farmer, and J.-L. Wolfender Spatial and Temporal Dynamics of Jasmonate Synthesis and Accumulation in Arabidopsis in Response to Wounding J. Biol. Chem., June 13, 2008; 283(24): 16400 - 16407. [Abstract] [Full Text] [PDF]

				L. Katsir, A. L. Schilmiller, P. E. Staswick, S. Y. He, and G. A. Howe From the Cover: COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine PNAS, May 13, 2008; 105(19): 7100 - 7105. [Abstract] [Full Text] [PDF]

				K. Kazan and J. M. Manners Jasmonate Signaling: Toward an Integrated View Plant Physiology, April 1, 2008; 146(4): 1459 - 1468. [Full Text] [PDF]

				S. Mueller, B. Hilbert, K. Dueckershoff, T. Roitsch, M. Krischke, M. J. Mueller, and S. Berger General Detoxification and Stress Responses Are Mediated by Oxidized Lipids through TGA Transcription Factors in Arabidopsis PLANT CELL, March 1, 2008; 20(3): 768 - 785. [Abstract] [Full Text] [PDF]

				J. Browse and G. A. Howe New Weapons and a Rapid Response against Insect Attack Plant Physiology, March 1, 2008; 146(3): 832 - 838. [Full Text] [PDF]

				H. S. Chung, A. J.K. Koo, X. Gao, S. Jayanty, B. Thines, A. D. Jones, and G. A. Howe Regulation and Function of Arabidopsis JASMONATE ZIM-Domain Genes in Response to Wounding and Herbivory Plant Physiology, March 1, 2008; 146(3): 952 - 964. [Abstract] [Full Text] [PDF]

				A. Santner and M. Estelle The JAZ Proteins Link Jasmonate Perception with Transcriptional Changes PLANT CELL, December 1, 2007; 19(12): 3839 - 3842. [Full Text] [PDF]