This Article Full Text Full Text (PDF) All Versions of this Article: 19/1/148    most recent tpc.106.044495v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Besseau, S. Articles by Legrand, M. Search for Related Content PubMed PubMed Citation Articles by Besseau, S. Articles by Legrand, M. Agricola Articles by Besseau, S. Articles by Legrand, M.

Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant Growth

^a Institut de Biologie Moléculaire des Plantes, Laboratoire Propre du Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Conventionné à l'Université Louis Pasteur, 67000 Strasbourg, France
^b Laboratoire de Chimie Biologique, Unité Mixte de Recherche 206, Institut National de la Recherche Agronomique–Institut National Agronomique, 78850 Thiverval-Grignon, France

² To whom correspondence should be addressed. E-mail michel.legrand{at}ibmp-ulp.u-strasbg.fr; fax 33-388-614442.

In Arabidopsis thaliana, silencing of hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), a lignin biosynthetic gene, results in a strong reduction of plant growth. We show that, in HCT-silenced plants, lignin synthesis repression leads to the redirection of the metabolic flux into flavonoids through chalcone synthase activity. Several flavonol glycosides and acylated anthocyanin were shown to accumulate in higher amounts in silenced plants. By contrast, sinapoylmalate levels were barely affected, suggesting that the synthesis of that phenylpropanoid compound might be HCT-independent. The growth phenotype of HCT-silenced plants was shown to be controlled by light and to depend on chalcone synthase expression. Histochemical analysis of silenced stem tissues demonstrated altered tracheary elements. The level of plant growth reduction of HCT-deficient plants was correlated with the inhibition of auxin transport. Suppression of flavonoid accumulation by chalcone synthase repression in HCT-deficient plants restored normal auxin transport and wild-type plant growth. By contrast, the lignin structure of the plants simultaneously repressed for HCT and chalcone synthase remained as severely altered as in HCT-silenced plants, with a large predominance of nonmethoxylated H units. These data demonstrate that the reduced size phenotype of HCT-silenced plants is not due to the alteration of lignin synthesis but to flavonoid accumulation.

This article has been cited by other articles:

				T. Bashandy, L. Taconnat, J.-P. Renou, Y. Meyer, and J.-P. Reichheld Accumulation of Flavonoids in an ntra ntrb Mutant Leads to Tolerance to UV-C Mol Plant, October 29, 2008; (2008) ssn065v1. [Abstract] [Full Text] [PDF]

				A. Fait, K. Hanhineva, R. Beleggia, N. Dai, I. Rogachev, V. J. Nikiforova, A. R. Fernie, and A. Aharoni Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit Development Plant Physiology, October 1, 2008; 148(2): 730 - 750. [Abstract] [Full Text] [PDF]

				T. Kamada-Nobusada, M. Hayashi, M. Fukazawa, H. Sakakibara, and M. Nishimura A Putative Peroxisomal Polyamine Oxidase, AtPAO4, is Involved in Polyamine Catabolism in Arabidopsis thaliana Plant Cell Physiol., September 1, 2008; 49(9): 1272 - 1282. [Abstract] [Full Text] [PDF]

				M. Peng, D. Hudson, A. Schofield, R. Tsao, R. Yang, H. Gu, Y.-M. Bi, and Steven. J. Rothstein Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene J. Exp. Bot., August 1, 2008; 59(11): 2933 - 2944. [Abstract] [Full Text] [PDF]

				C. Bottcher, E. von Roepenack-Lahaye, J. Schmidt, C. Schmotz, S. Neumann, D. Scheel, and S. Clemens Metabolome Analysis of Biosynthetic Mutants Reveals a Diversity of Metabolic Changes and Allows Identification of a Large Number of New Compounds in Arabidopsis Plant Physiology, August 1, 2008; 147(4): 2107 - 2120. [Abstract] [Full Text] [PDF]

				J. Stout, E. Romero-Severson, M. O. Ruegger, and C. Chapple Semidominant Mutations in Reduced Epidermal Fluorescence 4 Reduce Phenylpropanoid Content in Arabidopsis Genetics, April 1, 2008; 178(4): 2237 - 2251. [Abstract] [Full Text] [PDF]

				H. D. Coleman, J.-Y. Park, R. Nair, C. Chapple, and S. D. Mansfield RNAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism PNAS, March 18, 2008; 105(11): 4501 - 4506. [Abstract] [Full Text] [PDF]

				S. Moco, E. Capanoglu, Y. Tikunov, R. J. Bino, D. Boyacioglu, R. D. Hall, J. Vervoort, and R. C. H. De Vos Tissue specialization at the metabolite level is perceived during the development of tomato fruit J. Exp. Bot., December 7, 2007; (2007) erm271v1. [Abstract] [Full Text] [PDF]

				B. Dombrecht, G. P. Xue, S. J. Sprague, J. A. Kirkegaard, J. J. Ross, J. B. Reid, G. P. Fitt, N. Sewelam, P. M. Schenk, J. M. Manners, et al. MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis PLANT CELL, July 1, 2007; 19(7): 2225 - 2245. [Abstract] [Full Text] [PDF]