First published online December 9, 2005; 10.1105/tpc.105.037069
The Plant Cell 18:146-158 (2006)
© 2006 American Society of Plant Biologists
The Serine/Arginine-Rich Protein Family in Rice Plays Important Roles in Constitutive and Alternative Splicing of Pre-mRNA[W]
Masayuki Isshiki,
Ayako Tsumoto and
Ko Shimamoto1
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan
1 To whom correspondence should be addressed. E-mail simamoto{at}bs.naist.jp; fax 81-743-72-5509.
Ser/Arg-rich (SR) proteins play important roles in the constitutive and alternative splicing of pre-mRNA. We isolated 20 rice (Oryza sativa) genes encoding SR proteins, of which six contain plant-specific characteristics. To determine whether SR proteins modulate splicing efficiency and alternative splicing of pre-mRNA in rice, we used transient assays in rice protoplasts by cotransformation of SR protein genes with the rice Waxyb (Wxb)-ß-glucuronidase fusion gene. The results showed that plant-specific RSp29 and RSZp23, an SR protein homologous to human 9G8, enhanced splicing and altered the alternative 5' splice sites of Wxb intron 1. The resulting splicing pattern was unique to each SR protein; RSp29 stimulated splicing at the distal site, and RSZp23 enhanced splicing at the proximal site. Results of domain-swapping experiments between plant-specific RSp29 and SCL26, which is a homolog of human SC35, showed the importance of RNA recognition motif 1 and the Arg/Ser-rich (RS) domain for the enhancement of splicing efficiencies. Overexpression of plant-specific RSZ36 and SRp33b, a homolog of human ASF/SF2, in transgenic rice changed the alternative splicing patterns of their own pre-mRNAs and those of other SR proteins. These results show that SR proteins play important roles in constitutive and alternative splicing of rice pre-mRNA.
This article has been cited by other articles:
|
|
|
|
 
S. Bhatia, S. N. Gangappa, R. Kushwaha, S. Kundu, and S. Chattopadhyay
SHORT HYPOCOTYL IN WHITE LIGHT1, a Serine-Arginine-Aspartate-Rich Protein in Arabidopsis, Acts as a Negative Regulator of Photomorphogenic Growth
Plant Physiology,
May 1, 2008;
147(1):
169 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Tanabe, K. Yoshimura, A. Kimura, Y. Yabuta, and S. Shigeoka
Differential Expression of Alternatively Spliced mRNAs of Arabidopsis SR Protein Homologs, atSR30 and atSR45a, in Response to Environmental Stress
Plant Cell Physiol.,
July 1, 2007;
48(7):
1036 - 1049.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ner-Gaon, N. Leviatan, E. Rubin, and R. Fluhr
Comparative Cross-Species Alternative Splicing in Plants
Plant Physiology,
July 1, 2007;
144(3):
1632 - 1641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Niu, W. Zheng, B.-R. Lu, G. Ren, W. Huang, S. Wang, J. Liu, Z. Tang, D. Luo, Y. Wang, et al.
An Unusual Posttranscriptional Processing in Two Betaine Aldehyde Dehydrogenase Loci of Cereal Crops Directed by Short, Direct Repeats in Response to Stress Conditions
Plant Physiology,
April 1, 2007;
143(4):
1929 - 1942.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-J. Han, K.-H. Jung, G. Yi, D.-Y. Lee, and G. An
Rice Immature Pollen 1 (RIP1) is a Regulator of Late Pollen Development
Plant Cell Physiol.,
November 1, 2006;
47(11):
1457 - 1472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Tillemans, I. Leponce, G. Rausin, L. Dispa, and P. Motte
Insights into Nuclear Organization in Plants as Revealed by the Dynamic Distribution of Arabidopsis SR Splicing Factors
PLANT CELL,
November 1, 2006;
18(11):
3218 - 3234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kalyna, S. Lopato, V. Voronin, and A. Barta
Evolutionary conservation and regulation of particular alternative splicing events in plant SR proteins
Nucleic Acids Res.,
September 11, 2006;
34(16):
4395 - 4405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Iida and M. Go
Survey of Conserved Alternative Splicing Events of mRNAs Encoding SR Proteins in Land Plants
Mol. Biol. Evol.,
May 1, 2006;
23(5):
1085 - 1094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
