This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 17/9/2486    most recent tpc.105.033985v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Nakayama, N. Articles by Irish, V. F. Search for Related Content PubMed PubMed Citation Articles by Nakayama, N. Articles by Irish, V. F. Agricola Articles by Nakayama, N. Articles by Irish, V. F.

Gene Trap Lines Define Domains of Gene Regulation in Arabidopsis Petals and Stamens

^a Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104
^b Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

¹ To whom correspondence should be addressed. E-mail vivian.irish{at}yale.edu; fax 203-432-5711.

To identify genes involved in Arabidopsis thaliana petal and stamen organogenesis, we used a gene trap approach to examine the patterns of reporter expression at each stage of flower development of 1765 gene trap lines. In 80 lines, the reporter gene showed petal- and/or stamen-specific expression or lack of expression, or expression in distinct patterns within the petals and/or the stamens, including distinct suborgan domains of expression, such as tissue-specific lines marking epidermis and vasculature, as well as lines demarcating the proximodistal or abaxial/adaxial axes of the organs. Interestingly, reporter gene expression was typically restricted along the proximodistal axis of petals and stamens, indicating the importance of this developmental axis in patterning of gene expression domains in these organs. We identified novel domains of gene expression along the axis marking the midregion of the petals and apical and basal parts of the anthers. Most of the genes tagged in these 80 lines were identified, and their possible functions in petal and/or stamen differentiation are discussed. We also scored the floral phenotypes of the 1765 gene trap lines and recovered two mutants affecting previously uncharacterized genes. In addition to revealing common domains of gene expression, the gene trap lines reported here provide both useful markers and valuable starting points for reverse genetic analyses of the differentiation pathways in petal and stamen development.

This article has been cited by other articles:

				C. Fourquin, M. Vinauger-Douard, P. Chambrier, A. Berne-Dedieu, and C. P. Scutt Functional Conservation between CRABS CLAW Orthologues from Widely Diverged Angiosperms Ann. Bot., September 1, 2007; 100(3): 651 - 657. [Abstract] [Full Text] [PDF]

				S. R. Yadav, K. Prasad, and U. Vijayraghavan Divergent Regulatory OsMADS2 Functions Control Size, Shape and Differentiation of the Highly Derived Rice Floret Second-Whorl Organ Genetics, May 1, 2007; 176(1): 283 - 294. [Abstract] [Full Text] [PDF]

				S. Nagawa, S. Sawa, S. Sato, T. Kato, S. Tabata, and H. Fukuda Gene Trapping in Arabidopsis Reveals Genes Involved in Vascular Development Plant Cell Physiol., October 1, 2006; 47(10): 1394 - 1405. [Abstract] [Full Text] [PDF]

				L. I. A. Calderon-Villalobos, C. Kuhnle, H. Li, M. Rosso, B. Weisshaar, and C. Schwechheimer LucTrap Vectors Are Tools to Generate Luciferase Fusions for the Quantification of Transcript and Protein Abundance in Vivo. Plant Physiology, May 1, 2006; 141(1): 3 - 14. [Abstract] [Full Text] [PDF]

				S. K. Palaniswamy, S. James, H. Sun, R. S. Lamb, R. V. Davuluri, and E. Grotewold AGRIS and AtRegNet. A Platform to Link cis-Regulatory Elements and Transcription Factors into Regulatory Networks Plant Physiology, March 1, 2006; 140(3): 818 - 829. [Abstract] [Full Text] [PDF]