This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 17/9/2454    most recent tpc.105.031203v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Sarnowski, T. J. Articles by Jerzmanowski, A. Search for Related Content PubMed PubMed Citation Articles by Sarnowski, T. J. Articles by Jerzmanowski, A. Agricola Articles by Sarnowski, T. J. Articles by Jerzmanowski, A.

SWI3 Subunits of Putative SWI/SNF Chromatin-Remodeling Complexes Play Distinct Roles during Arabidopsis Development

Tomasz J. Sarnowski^a,1, Gabino Ríos^b,1,2, Jan Jásik^b,1, Szymon wieewski^a, Szymon Kaczanowski^a, Yong Li^b, Aleksandra Kwiatkowska^c, Katarzyna Pawlikowska^a, Marta Kobia^c, Piotr Kobia^c, Csaba Koncz^b and Andrzej Jerzmanowski^a,c,3

^a Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
^b Max-Planck-Institut für Züchtungsforschung, D-50829 Köln, Germany
^c Laboratory of Plant Molecular Biology, Warsaw University, 02-106 Warsaw, Poland

³ To whom correspondence should be addressed. E-mail andyj{at}ibb.waw.pl; fax 4822-6584636.

SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin-remodeling complexes mediate ATP-dependent alterations of DNA–histone contacts. The minimal functional core of conserved SWI/SNF complexes consists of a SWI2/SNF2 ATPase, SNF5, SWP73, and a pair of SWI3 subunits. Because of early duplication of the SWI3 gene family in plants, Arabidopsis thaliana encodes four SWI3-like proteins that show remarkable functional diversification. Whereas ATSWI3A and ATSWI3B form homodimers and heterodimers and interact with BSH/SNF5, ATSWI3C, and the flowering regulator FCA, ATSWI3D can only bind ATSWI3B in yeast two-hybrid assays. Mutations of ATSWI3A and ATSWI3B arrest embryo development at the globular stage. By a possible imprinting effect, the atswi3b mutations result in death for approximately half of both macrospores and microspores. Mutations in ATSWI3C cause semidwarf stature, inhibition of root elongation, leaf curling, aberrant stamen development, and reduced fertility. Plants carrying atswi3d mutations display severe dwarfism, alterations in the number and development of flower organs, and complete male and female sterility. These data indicate that, by possible contribution to the combinatorial assembly of different SWI/SNF complexes, the ATSWI3 proteins perform nonredundant regulatory functions that affect embryogenesis and both the vegetative and reproductive phases of plant development.

This article has been cited by other articles:

				X. Tang, A. Hou, M. Babu, V. Nguyen, L. Hurtado, Q. Lu, J. C. Reyes, A. Wang, W. A. Keller, J. J. Harada, et al. The Arabidopsis BRAHMA Chromatin-Remodeling ATPase Is Involved in Repression of Seed Maturation Genes in Leaves Plant Physiology, July 1, 2008; 147(3): 1143 - 1157. [Abstract] [Full Text] [PDF]

				S. Bezhani, C. Winter, S. Hershman, J. D. Wagner, J. F. Kennedy, C. S. Kwon, J. Pfluger, Y. Su, and D. Wagner Unique, Shared, and Redundant Roles for the Arabidopsis SWI/SNF Chromatin Remodeling ATPases BRAHMA and SPLAYED PLANT CELL, February 1, 2007; 19(2): 403 - 416. [Abstract] [Full Text] [PDF]

				H. MacWilliams, K. Doquang, R. Pedrola, G. Dollman, D. Grassi, T. Peis, A. Tsang, and A. Ceccarelli A retinoblastoma ortholog controls stalk/spore preference in Dictyostelium Development, April 1, 2006; 133(7): 1287 - 1297. [Abstract] [Full Text] [PDF]

				R. B. Meagher, R. B. Deal, M. K. Kandasamy, and E. C. McKinney Nuclear Actin-Related Proteins as Epigenetic Regulators of Development Plant Physiology, December 1, 2005; 139(4): 1576 - 1585. [Full Text] [PDF]