Plant Cell Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 19, 2005; 10.1105/tpc.104.026690

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
17/2/616    most recent
tpc.104.026690v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (65)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Staswick, P. E.
Right arrow Articles by Suza, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Staswick, P. E.
Right arrow Articles by Suza, W.
Agricola
Right arrow Articles by Staswick, P. E.
Right arrow Articles by Suza, W.
The Plant Cell 17:616-627 (2005)
© 2005 American Society of Plant Biologists

Characterization of an Arabidopsis Enzyme Family That Conjugates Amino Acids to Indole-3-Acetic Acid{boxw}

Paul E. Staswicka,1, Bogdan Serbanb, Martha Rowea, Iskender Tiryakia,2, Marién T. Maldonadoa,3, Mitsa C. Maldonadoa,3 and Walter Suzaa

a Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska 68583
b Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68583

1 To whom correspondence should be addressed. E-mail pstaswick1{at}unl.edu; fax 402-472-7904.

Substantial evidence indicates that amino acid conjugates of indole-3-acetic acid (IAA) function in auxin homeostasis, yet the plant enzymes involved in their biosynthesis have not been identified. We tested whether several Arabidopsis thaliana enzymes that are related to the auxin-induced soybean (Glycine max) GH3 gene product synthesize IAA–amino acid conjugates. In vitro reactions with six recombinant GH3 enzymes produced IAA conjugates with several amino acids, based on thin layer chromatography. The identity of the Ala, Asp, Phe, and Trp conjugates was verified by gas chromatography–mass spectrometry. Insertional mutations in GH3.1, GH3.2, GH3.5, and GH3.17 resulted in modestly increased sensitivity to IAA in seedling root. Overexpression of GH3.6 in the activation-tagged mutant dfl1-D did not significantly alter IAA level but resulted in 3.2- and 4.5-fold more IAA-Asp than in wild-type seedlings and mature leaves, respectively. In addition to IAA, dfl1-D was less sensitive to indole-3-butyric acid and naphthaleneacetic acid, consistent with the fact that GH3.6 was active on each of these auxins. By contrast, GH3.6 and the other five enzymes tested were inactive on halogenated auxins, and dfl1-D was not resistant to these. This evidence establishes that several GH3 genes encode IAA-amido synthetases, which help to maintain auxin homeostasis by conjugating excess IAA to amino acids.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Y. Yang, R. Xu, C.-j. Ma, A. C. Vlot, D. F. Klessig, and E. Pichersky
Inactive Methyl Indole-3-Acetic Acid Ester Can Be Hydrolyzed and Activated by Several Esterases Belonging to the AtMES Esterase Family of Arabidopsis
Plant Physiology, July 1, 2008; 147(3): 1034 - 1045.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
I. A. Paponov, M. Paponov, W. Teale, M. Menges, S. Chakrabortee, J. A.H. Murray, and K. Palme
Comprehensive Transcriptome Analysis of Auxin Responses in Arabidopsis
Mol Plant, March 1, 2008; 1(2): 321 - 337.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Kienow, K. Schneider, M. Bartsch, H.-P. Stuible, H. Weng, O. Miersch, C. Wasternack, and E. Kombrink
Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana
J. Exp. Bot., February 10, 2008; (2008) erm325v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Ding, Y. Cao, L. Huang, J. Zhao, C. Xu, X. Li, and S. Wang
Activation of the Indole-3-Acetic Acid-Amido Synthetase GH3-8 Suppresses Expansin Expression and Promotes Salicylate- and Jasmonate-Independent Basal Immunity in Rice
PLANT CELL, January 1, 2008; 20(1): 228 - 240.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C. Sungur, S. Miller, J. Bergholz, R. C. Hoye, R. G. Brisbois, and P. Overvoorde
The Small Molecule 2-Furylacrylic Acid Inhibits Auxin-Mediated Responses in Arabidopsis thaliana
Plant Cell Physiol., December 1, 2007; 48(12): 1693 - 1701.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. K. Biswas, C. Ooura, K. Higuchi, Y. Miyazaki, V. Van Nguyen, A. Rahman, H. Uchimiya, T. Kiyosue, T. Koshiba, A. Tanaka, et al.
Genetic Characterization of Mutants Resistant to the Antiauxin p-Chlorophenoxyisobutyric Acid Reveals That AAR3, a Gene Encoding a DCN1-Like Protein, Regulates Responses to the Synthetic Auxin 2,4-Dichlorophenoxyacetic Acid in Arabidopsis Roots
Plant Physiology, November 1, 2007; 145(3): 773 - 785.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
C. Wasternack
Jasmonates: An Update on Biosynthesis, Signal Transduction and Action in Plant Stress Response, Growth and Development
Ann. Bot., October 1, 2007; 100(4): 681 - 697.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Zhang, Q. Li, Z. Li, P. E. Staswick, M. Wang, Y. Zhu, and Z. He
Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin Signaling during Arabidopsis-Pseudomonas syringae Interaction
Plant Physiology, October 1, 2007; 145(2): 450 - 464.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J.-E. Park, P. J. Seo, A.-K. Lee, J.-H. Jung, Y.-S. Kim, and C.-M. Park
An Arabidopsis GH3 Gene, Encoding an Auxin-Conjugating Enzyme, Mediates Phytochrome B-Regulated Light Signals in Hypocotyl Growth
Plant Cell Physiol., August 1, 2007; 48(8): 1236 - 1241.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Shin, A. Y. Burch, K. A. Huppert, S. B. Tiwari, A. S. Murphy, T. J. Guilfoyle, and D. P. Schachtman
The Arabidopsis Transcription Factor MYB77 Modulates Auxin Signal Transduction
PLANT CELL, August 1, 2007; 19(8): 2440 - 2453.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Nobuta, R.A. Okrent, M. Stoutemyer, N. Rodibaugh, L. Kempema, M.C. Wildermuth, and R.W. Innes
The GH3 Acyl Adenylase Family Member PBS3 Regulates Salicylic Acid-Dependent Defense Responses in Arabidopsis
Plant Physiology, June 1, 2007; 144(2): 1144 - 1156.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-E. Park, J.-Y. Park, Y.-S. Kim, P. E. Staswick, J. Jeon, J. Yun, S.-Y. Kim, J. Kim, Y.-H. Lee, and C.-M. Park
GH3-mediated Auxin Homeostasis Links Growth Regulation with Stress Adaptation Response in Arabidopsis
J. Biol. Chem., March 30, 2007; 282(13): 10036 - 10046.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Ren, K. K. Mandadi, A. L. Boedeker, K. S. Rathore, and T. D. McKnight
Regulation of Telomerase in Arabidopsis by BT2, an Apparent Target of TELOMERASE ACTIVATOR1
PLANT CELL, January 1, 2007; 19(1): 23 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M.-a. Ohto, S. Hayashi, S. Sawa, A. Hashimoto-Ohta, and K. Nakamura
Involvement of HLS1 in Sugar and Auxin Signaling in Arabidopsis Leaves
Plant Cell Physiol., December 1, 2006; 47(12): 1603 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-H. Kang, L. Wang, A. Giri, and I. T. Baldwin
Silencing Threonine Deaminase and JAR4 in Nicotiana attenuata Impairs Jasmonic Acid-Isoleucine-Mediated Defenses against Manduca sexta
PLANT CELL, November 1, 2006; 18(11): 3303 - 3320.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y.-S. Kim, S.-G. Kim, J.-E. Park, H.-Y. Park, M.-H. Lim, N.-H. Chua, and C.-M. Park
A Membrane-Bound NAC Transcription Factor Regulates Cell Division in Arabidopsis
PLANT CELL, November 1, 2006; 18(11): 3132 - 3144.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Cao, H. Cheng, W. Wu, H. M. Soo, and J. Peng
Gibberellin Mobilizes Distinct DELLA-Dependent Transcriptomes to Regulate Seed Germination and Floral Development in Arabidopsis
Plant Physiology, October 1, 2006; 142(2): 509 - 525.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. van der Graaff, R. Schwacke, A. Schneider, M. Desimone, U.-I. Flugge, and R. Kunze
Transcription Analysis of Arabidopsis Membrane Transporters and Hormone Pathways during Developmental and Induced Leaf Senescence
Plant Physiology, June 1, 2006; 141(2): 776 - 792.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Che, S. Lall, D. Nettleton, and S. H. Howell
Gene Expression Programs during Shoot, Root, and Callus Development in Arabidopsis Tissue Culture
Plant Physiology, June 1, 2006; 141(2): 620 - 637.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. A. Esmon, A. G. Tinsley, K. Ljung, G. Sandberg, L. B. Hearne, and E. Liscum
A gradient of auxin and auxin-dependent transcription precedes tropic growth responses
PNAS, January 3, 2006; 103(1): 236 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. H. Yang, S. J. Han, E. K. Yoon, and W. S. Lee
'Evidence of an auxin signal pathway, microRNA167-ARF8-GH3, and its response to exogenous auxin in cultured rice cells'.
Nucleic Acids Res., January 1, 2006; 34(6): 1892 - 1899.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Sorin, L. Negroni, T. Balliau, H. Corti, M.-P. Jacquemot, M. Davanture, G. Sandberg, M. Zivy, and C. Bellini
Proteomic Analysis of Different Mutant Genotypes of Arabidopsis Led to the Identification of 11 Proteins Correlating with Adventitious Root Development
Plant Physiology, January 1, 2006; 140(1): 349 - 364.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Yoshida, H. Kuriyama, and H. Fukuda
Inhibition of Transdifferentiation into Tracheary Elements by Polar Auxin Transport Inhibitors Through Intracellular Auxin Depletion
Plant Cell Physiol., December 1, 2005; 46(12): 2019 - 2028.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Vanneste, B. De Rybel, G. T.S. Beemster, K. Ljung, I. De Smet, G. Van Isterdael, M. Naudts, R. Iida, W. Gruissem, M. Tasaka, et al.
Cell Cycle Progression in the Pericycle Is Not Sufficient for SOLITARY ROOT/IAA14-Mediated Lateral Root Initiation in Arabidopsis thaliana
PLANT CELL, November 1, 2005; 17(11): 3035 - 3050.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. Qin, H. Gu, Y. Zhao, Z. Ma, G. Shi, Y. Yang, E. Pichersky, H. Chen, M. Liu, Z. Chen, et al.
An Indole-3-Acetic Acid Carboxyl Methyltransferase Regulates Arabidopsis Leaf Development
PLANT CELL, October 1, 2005; 17(10): 2693 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
MicroRNAs Regulate Auxin Homeostasis and Plant Development
PLANT CELL, May 1, 2005; 17(5): 1335 - 1338.
[Full Text] [PDF]

Home page
 Plant CellHome page
A. C. Mallory, D. P. Bartel, and B. Bartel
MicroRNA-Directed Regulation of Arabidopsis AUXIN RESPONSE FACTOR17 Is Essential for Proper Development and Modulates Expression of Early Auxin Response Genes
PLANT CELL, May 1, 2005; 17(5): 1360 - 1375.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Sorin, J. D. Bussell, I. Camus, K. Ljung, M. Kowalczyk, G. Geiss, H. McKhann, C. Garcion, H. Vaucheret, G. Sandberg, et al.
Auxin and Light Control of Adventitious Rooting in Arabidopsis Require ARGONAUTE1
PLANT CELL, May 1, 2005; 17(5): 1343 - 1359.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Inukai, T. Sakamoto, M. Ueguchi-Tanaka, Y. Shibata, K. Gomi, I. Umemura, Y. Hasegawa, M. Ashikari, H. Kitano, and M. Matsuoka
Crown rootless1, Which Is Essential for Crown Root Formation in Rice, Is a Target of an AUXIN RESPONSE FACTOR in Auxin Signaling
PLANT CELL, May 1, 2005; 17(5): 1387 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. W. WOODWARD and B. BARTEL
Auxin: Regulation, Action, and Interaction
Ann. Bot., April 1, 2005; 95(5): 707 - 735.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2005 by the American Society of Plant Biologists