First published online November 11, 2005; 10.1105/tpc.105.035659
The Plant Cell 17:3470-3488 (2005)
© 2005 American Society of Plant Biologists
OPEN ACCESS ARTICLE
AREB1 Is a Transcription Activator of Novel ABRE-Dependent ABA Signaling That Enhances Drought Stress Tolerance in Arabidopsis[W],[OA]
Yasunari Fujitaa,
Miki Fujitab,c,
Rie Satoha,c,
Kyonoshin Maruyamaa,
Mohammad M. Parveza,
Motoaki Sekib,d,
Keiichiro Hiratsuc,e,
Masaru Ohme-Takagic,e,
Kazuo Shinozakib,c,d and
Kazuko Yamaguchi-Shinozakia,c,f,1
a Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan
b Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074, Japan
c Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
d Plant Functional Genomics Group, RIKEN Genomic Sciences Center, Yokohama, Kanagawa 230-0045, Japan
e Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Central 4, Tsukuba, Ibaraki 305-8562, Japan
f Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan
1 To whom correspondence should be addressed. E-mail kazukoys{at}jircas.affrc.go.jp; fax 81-29-838-6643.
ABSCISIC ACID–RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) (i.e., ABF2) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)–responsive element (ABRE) motif in the promoter region of ABA-inducible genes. Here, we show that expression of the intact AREB1 gene on its own is insufficient to lead to expression of downstream genes under normal growth conditions. To overcome the masked transactivation activity of AREB1, we created an activated form of AREB1 (AREB1 QT). AREB1QT-overexpressing plants showed ABA hypersensitivity and enhanced drought tolerance, and eight genes with two or more ABRE motifs in the promoter regions in two groups were greatly upregulated: late embryogenesis abundant class genes and ABA- and drought stress–inducible regulatory genes. By contrast, an areb1 null mutant and a dominant loss-of-function mutant of AREB1 (AREB1:RD) with a repression domain exhibited ABA insensitivity. Furthermore, AREB1:RD plants displayed reduced survival under dehydration, and three of the eight greatly upregulated genes were downregulated, including genes for linker histone H1 and AAA ATPase, which govern gene expression and multiple cellular activities through protein folding, respectively. Thus, these data suggest that AREB1 regulates novel ABRE-dependent ABA signaling that enhances drought tolerance in vegetative tissues.
This article has been cited by other articles:
|
|
|
|
 
K. Nakashima, Y. Ito, and K. Yamaguchi-Shinozaki
Transcriptional Regulatory Networks in Response to Abiotic Stresses in Arabidopsis and Grasses
Plant Physiology,
January 1, 2009;
149(1):
88 - 95.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Qin, Y. Sakuma, L.-S. P. Tran, K. Maruyama, S. Kidokoro, Y. Fujita, M. Fujita, T. Umezawa, Y. Sawano, K.-i. Miyazono, et al.
Arabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress-Responsive Gene Expression
PLANT CELL,
June 1, 2008;
20(6):
1693 - 1707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zhang, B. Wollenweber, D. Jiang, F. Liu, and J. Zhao
Water deficits and heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor
J. Exp. Bot.,
March 1, 2008;
59(4):
839 - 848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nijhawan, M. Jain, A. K. Tyagi, and J. P. Khurana
Genomic Survey and Gene Expression Analysis of the Basic Leucine Zipper Transcription Factor Family in Rice
Plant Physiology,
February 1, 2008;
146(2):
333 - 350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-S. P. Tran, T. Urao, F. Qin, K. Maruyama, T. Kakimoto, K. Shinozaki, and K. Yamaguchi-Shinozaki
Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis
PNAS,
December 18, 2007;
104(51):
20623 - 20628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kant, S. Kant, M. Gordon, R. Shaked, and S. Barak
STRESS RESPONSE SUPPRESSOR1 and STRESS RESPONSE SUPPRESSOR2, Two DEAD-Box RNA Helicases That Attenuate Arabidopsis Responses to Multiple Abiotic Stresses
Plant Physiology,
November 1, 2007;
145(3):
814 - 830.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Zhu, X.-C. Yu, X.-J. Wang, R. Zhao, Y. Li, R.-C. Fan, Y. Shang, S.-Y. Du, X.-F. Wang, F.-Q. Wu, et al.
Two Calcium-Dependent Protein Kinases, CPK4 and CPK11, Regulate Abscisic Acid Signal Transduction in Arabidopsis
PLANT CELL,
October 1, 2007;
19(10):
3019 - 3036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ruttink, M. Arend, K. Morreel, V. Storme, S. Rombauts, J. Fromm, R. P. Bhalerao, W. Boerjan, and A. Rohde
A Molecular Timetable for Apical Bud Formation and Dormancy Induction in Poplar
PLANT CELL,
August 1, 2007;
19(8):
2370 - 2390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Shinozaki and K. Yamaguchi-Shinozaki
Gene networks involved in drought stress response and tolerance
J. Exp. Bot.,
January 1, 2007;
58(2):
221 - 227.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Nilson and S. M. Assmann
The Control of Transpiration. Insights from Arabidopsis
Plant Physiology,
January 1, 2007;
143(1):
19 - 27.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Stone, L. A. Williams, L. M. Farmer, R. D. Vierstra, and J. Callis
KEEP ON GOING, a RING E3 Ligase Essential for Arabidopsis Growth and Development, Is Involved in Abscisic Acid Signaling
PLANT CELL,
December 1, 2006;
18(12):
3415 - 3428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Furihata, K. Maruyama, Y. Fujita, T. Umezawa, R. Yoshida, K. Shinozaki, and K. Yamaguchi-Shinozaki
Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1
PNAS,
February 7, 2006;
103(6):
1988 - 1993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
