This Article Full Text Full Text (PDF) All Versions of this Article: 17/11/3051    most recent tpc.105.034413v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Lee, K. J.D. Articles by Knox, J. P. Search for Related Content PubMed PubMed Citation Articles by Lee, K. J.D. Articles by Knox, J. P. Agricola Articles by Lee, K. J.D. Articles by Knox, J. P.

Arabinogalactan Proteins Are Required for Apical Cell Extension in the Moss Physcomitrella patens

^a Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
^b Department of Biology, Washington University, St. Louis, Missouri 63130-4899
^c Plant Cell Biology Research Centre and Cooperative Research Centre for Bioproducts, School of Botany, University of Melbourne, 3010 Victoria, Australia

² To whom correspondence should be addressed. E-mail j.p.knox{at}leeds.ac.uk; fax 44-113-3433144.

Cell biological, structural, and genetic approaches have demonstrated the presence of arabinogalactan proteins (AGPs) in the moss Physcomitrella patens and provided evidence for their function in cell expansion and specifically in the extension of apical tip-growing cells. Inhibitor studies indicated that apical cell expansion in P. patens is blocked by synthetic AGP binding ß-glucosyl Yariv reagent (ßGlcYR). The anti-(15)--L-arabinan monoclonal antibody LM6 binds to some AGPs in P. patens, to all plasma membranes, and to the cell wall surface at the most apical region of growing protonemal filaments. Moreover, LM6 labeling of cell walls at the tips of apical cells of P. patens was abolished in the presence of ßGlcYR, suggesting that the localized movement of AGPs from the plasma membrane to the cell wall is a component of the mechanism of tip growth. Biochemical and bioinformatic analyses were used to identify seven P. patens ESTs encoding putative AGP core proteins from homology with Arabidopsis thaliana, Brassica napus, and Oryza sativa sequences and from peptide fragments isolated from ßGlcYR-precipitated AGPs. Gene knockout by homologous recombination of one of these genes, P. patens AGP1, encoding a classical AGP core protein, resulted in reduced cell lengths in protonemal filaments, indicating a role for AGP1 in apical cell expansion in P. patens.

This article has been cited by other articles:

				N. Q. Phan, S.-J. Kim, and D. C. Bassham Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole Mol Plant, November 1, 2008; 1(6): 961 - 976. [Abstract] [Full Text] [PDF]

				M. J Pena, A. G. Darvill, S. Eberhard, W. S York, and M. A O'Neill Moss and liverwort xyloglucans contain galacturonic acid and are structurally distinct from the xyloglucans synthesized by hornworts and vascular plants Glycobiology, November 1, 2008; 18(11): 891 - 904. [Abstract] [Full Text] [PDF]

				R. A. Chavez Montes, P. Ranocha, Y. Martinez, Z. Minic, L. Jouanin, M. Marquis, L. Saulnier, L. M. Fulton, C. S. Cobbett, F. Bitton, et al. Cell Wall Modifications in Arabidopsis Plants with Altered {alpha}-L-Arabinofuranosidase Activity Plant Physiology, May 1, 2008; 147(1): 63 - 77. [Abstract] [Full Text] [PDF]

				P.-F. Perroud and R. S. Quatrano BRICK1 Is Required for Apical Cell Growth in Filaments of the Moss Physcomitrella patens but Not for Gametophore Morphology PLANT CELL, February 1, 2008; 20(2): 411 - 422. [Abstract] [Full Text] [PDF]

				Y. Li, C. Smith, F. Corke, L. Zheng, Z. Merali, P. Ryden, P. Derbyshire, K. Waldron, and M. W. Bevan Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development in Arabidopsis thaliana PLANT CELL, August 1, 2007; 19(8): 2500 - 2515. [Abstract] [Full Text] [PDF]

				B. Menand, G. Calder, and L. Dolan Both chloronemal and caulonemal cells expand by tip growth in the moss Physcomitrella patens J. Exp. Bot., May 1, 2007; 58(7): 1843 - 1849. [Abstract] [Full Text] [PDF]

				J. Rosti, C. J. Barton, S. Albrecht, P. Dupree, M. Pauly, K. Findlay, K. Roberts, and G. J. Seifert UDP-Glucose 4-Epimerase Isoforms UGE2 and UGE4 Cooperate in Providing UDP-Galactose for Cell Wall Biosynthesis and Growth of Arabidopsis thaliana PLANT CELL, May 1, 2007; 19(5): 1565 - 1579. [Abstract] [Full Text] [PDF]

				T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi, M. A. O'Neill, and T. Ishii A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose Glycobiology, March 1, 2007; 17(3): 345 - 354. [Abstract] [Full Text] [PDF]

				J. M. Estevez, M. J. Kieliszewski, N. Khitrov, and C. Somerville Characterization of Synthetic Hydroxyproline-Rich Proteoglycans with Arabinogalactan Protein and Extensin Motifs in Arabidopsis Plant Physiology, October 1, 2006; 142(2): 458 - 470. [Abstract] [Full Text] [PDF]

				J. P. Moore, E. Nguema-Ona, L. Chevalier, G. G. Lindsey, W. F. Brandt, P. Lerouge, J. M. Farrant, and A. Driouich Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius Plant Physiology, June 1, 2006; 141(2): 651 - 662. [Abstract] [Full Text] [PDF]