Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online August 12, 2004; 10.1105/tpc.104.024919

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
16/9/2307    most recent
tpc.104.024919v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qiao, H.
Right arrow Articles by Xue, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qiao, H.
Right arrow Articles by Xue, Y.
Agricola
Right arrow Articles by Qiao, H.
Right arrow Articles by Xue, Y.
The Plant Cell 16:2307-2322 (2004)
© 2004 American Society of Plant Biologists

The F-Box Protein AhSLF-S2 Controls the Pollen Function of S-RNase–Based Self-Incompatibility

Hong Qiaoa,1, Fei Wanga,1, Lan Zhaoa,1, Junli Zhoua, Zhao Laia, Yansheng Zhanga, Timothy P. Robbinsb and Yongbiao Xuea,2

a Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
b Plant Science Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, United Kingdom

2 To whom correspondence should be addressed. E-mail ybxue{at}genetics.ac.cn; fax 86-10-6253-7814.

Recently, we have provided evidence that the polymorphic self-incompatibility (S) locus-encoded F-box (SLF) protein AhSLF-S2 plays a role in mediating a selective S-RNase destruction during the self-incompatible response in Antirrhinum hispanicum. To investigate its role further, we first transformed a transformation-competent artificial chromosome clone (TAC26) containing both AhSLF-S2 and AhS2-RNase into a self-incompatible (SI) line of Petunia hybrida. Molecular analyses showed that both genes are correctly expressed in pollen and pistil in four independent transgenic lines of petunia. Pollination tests indicated that all four lines became self-compatible because of the specific loss of the pollen function of SI. This alteration was transmitted stably into the T1 progeny. We then transformed AhSLF-S2 cDNA under the control of a tomato (Lycopersicon esculentum) pollen-specific promoter LAT52 into the self-incompatible petunia line. Molecular studies revealed that AhSLF-S2 is specifically expressed in pollen of five independent transgenic plants. Pollination tests showed that they also had lost the pollen function of SI. Importantly, expression of endogenous SLF or SLF-like genes was not altered in these transgenic plants. These results phenocopy a well-known phenomenon called competitive interaction whereby the presence of two different pollen S alleles within pollen leads to the breakdown of the pollen function of SI in several solanaceaous species. Furthermore, we demonstrated that AhSLF-S2 physically interacts with PhS3-RNase from the P. hybrida line used for transformation. Together with the recent demonstration of PiSLF as the pollen determinant in P. inflata, these results provide direct evidence that the polymorphic SLF including AhSLF-S2 controls the pollen function of S-RNase–based self-incompatibility.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Yang, U. C. Kalluri, S. Jawdy, L. E. Gunter, T. Yin, T. J. Tschaplinski, D. J. Weston, P. Ranjan, and G. A. Tuskan
The F-Box Gene Family Is Expanded in Herbaceous Annual Plants Relative to Woody Perennial Plants
Plant Physiology, November 1, 2008; 148(3): 1189 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
W.-Q. Yang, Y. Lai, M.-N. Li, W.-Y. Xu, and Y.-B. Xue
A Novel C2-Domain Phospholipid-Binding Protein, OsPBP1, Is Required for Pollen Fertility in Rice
Mol Plant, September 1, 2008; 1(5): 770 - 785.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
Z.-H. Hua, A. Fields, and T.-h. Kao
Biochemical Models for S-RNase-Based Self-Incompatibility
Mol Plant, July 1, 2008; 1(4): 575 - 585.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Wheeler and E. Newbigin
Expression of 10 S-Class SLF-like Genes in Nicotiana alata Pollen and Its Implications for Understanding the Pollen Factor of the S Locus
Genetics, December 1, 2007; 177(4): 2171 - 2180.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Hua, X. Meng, and T.-h. Kao
Comparison of Petunia inflata S-Locus F-Box Protein (Pi SLF) with Pi SLF Like Proteins Reveals Its Unique Function in S-RNase Based Self-Incompatibility
PLANT CELL, November 1, 2007; 19(11): 3593 - 3609.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Jain, A. Nijhawan, R. Arora, P. Agarwal, S. Ray, P. Sharma, S. Kapoor, A. K. Tyagi, and J. P. Khurana
F-Box Proteins in Rice. Genome-Wide Analysis, Classification, Temporal and Spatial Gene Expression during Panicle and Seed Development, and Regulation by Light and Abiotic Stress
Plant Physiology, April 1, 2007; 143(4): 1467 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
S. Dai, T. Chen, K. Chong, Y. Xue, S. Liu, and T. Wang
Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen
Mol. Cell. Proteomics, February 1, 2007; 6(2): 207 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Chen, V. J. Karplus, H. Ma, and X. W. Deng
Plant Biology Research Comes of Age in China
PLANT CELL, November 1, 2006; 18(11): 2855 - 2864.
[Full Text] [PDF]

Home page
 Plant CellHome page
Z. Hua and T.-h. Kao
Identification and Characterization of Components of a Putative Petunia S-Locus F-Box-Containing E3 Ligase Complex Involved in S-RNase-Based Self-Incompatibility
PLANT CELL, October 1, 2006; 18(10): 2531 - 2553.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Kong, M. Li, W. Yang, W. Xu, and Y. Xue
A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice
Plant Physiology, August 1, 2006; 141(4): 1376 - 1388.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
X. Qin, B. Liu, J. Soulard, D. Morse, and M. Cappadocia
Style-by-style analysis of two sporadic self-compatible Solanum chacoense lines supports a primary role for S-RNases in determining pollen rejection thresholds
J. Exp. Bot., June 1, 2006; 57(9): 2001 - 2013.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Sonneveld, K. R. Tobutt, S. P. Vaughan, and T. P. Robbins
Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype-Specific F-Box Gene
PLANT CELL, January 1, 2005; 17(1): 37 - 51.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Moon, G. Parry, and M. Estelle
The Ubiquitin-Proteasome Pathway and Plant Development
PLANT CELL, December 1, 2004; 16(12): 3181 - 3195.
[Full Text] [PDF]

Home page
 Plant CellHome page
B. McClure
S-RNase and SLF Determine S-Haplotype-Specific Pollen Recognition and Rejection
PLANT CELL, November 1, 2004; 16(11): 2840 - 2847.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2004 by the American Society of Plant Biologists