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First published online May 21, 2004; 10.1105/tpc.021634

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The Plant Cell 16:1589-1603 (2004)
© 2004 American Society of Plant Biologists

The Arabidopsis Rab GTPase RabA4b Localizes to the Tips of Growing Root Hair Cells

Mary L. Preussa, Jannie Sernaa, Tanya G. Falbelb, Sebastian Y. Bednarekb and Erik Nielsena,1

a Donald Danforth Plant Science Center, St. Louis, Missouri 63132
b Department of Biochemistry, University of Wisconsin–Madison, Madison, Wisconsin 53706

1 To whom correspondence should be addressed. E-mail enielsen{at}danforthcenter.org; fax 314-587-1381.

Spatial and temporal control of cell wall deposition plays a unique and critical role during growth and development in plants. To characterize membrane trafficking pathways involved in these processes, we have examined the function of a plant Rab GTPase, RabA4b, during polarized expansion in developing root hair cells. Whereas a small fraction of RabA4b cofractionated with Golgi membrane marker proteins, the majority of this protein labeled a unique membrane compartment that did not cofractionate with the previously characterized trans-Golgi network syntaxin proteins SYP41 and SYP51. An enhanced yellow fluorescent protein (EYFP)-RabA4b fusion protein specifically localizes to the tips of growing root hair cells in Arabidopsis thaliana. Tip-localized EYFP-RabA4b disappears in mature root hair cells that have stopped expanding, and polar localization of the EYFP-RabA4b is disrupted by latrunculin B treatment. Loss of tip localization of EYFP-RabA4b was correlated with inhibition of expansion; upon washout of the inhibitor, root hair expansion recovered only after tip localization of the EYFP-RabA4b compartments was reestablished. Furthermore, in mutants with defective root hair morphology, EYFP-RabA4b was improperly localized or was absent from the tips of root hair cells. We propose that RabA4b regulates membrane trafficking through a compartment involved in the polarized secretion of cell wall components in plant cells.




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