First published online April 14, 2004; 10.1105/tpc.017830
The Plant Cell 16:1178-1190 (2004)
© 2004 American Society of Plant Biologists
SPIRAL1 Encodes a Plant-Specific Microtubule-Localized Protein Required for Directional Control of Rapidly Expanding Arabidopsis Cells
Keiji Nakajima,
Ikuyo Furutani,
Hideki Tachimoto,
Hiroshige Matsubara and
Takashi Hashimoto1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan
1 To whom correspondence should be addressed. E-mail hasimoto{at}bs.naist.jp; fax 81-743-72-5529.
Highly organized interphase cortical microtubule (MT) arrays are essential for anisotropic growth of plant cells, yet little is known about the molecular mechanisms that establish and maintain the order of these arrays. The Arabidopsis thaliana spiral1 (spr1) mutant shows right-handed helical growth in roots and etiolated hypocotyls. Characterization of the mutant phenotypes suggested that SPR1 may control anisotropic cell expansion through MT-dependent processes. SPR1 was identified by map-based cloning and found to encode a small protein with unknown function. Proteins homologous to SPR1 occur specifically and ubiquitously in plants. Genetic complementation with green fluorescent protein fusion proteins indicated that the SPR1 protein colocalizes with cortical MTs and that both MT localization and cell expansion control are conferred by the conserved N- and C-terminal regions. Strong SPR1 expression was found in tissues undergoing rapid cell elongation. Plants overexpressing SPR1 showed enhanced resistance to an MT drug and increased hypocotyl elongation. These observations suggest that SPR1 is a plant-specific MT-localized protein required for the maintenance of growth anisotropy in rapidly elongating cells.
This article has been cited by other articles:
|
|
|
|
 
H. Buschmann and C. W. Lloyd
Arabidopsis Mutants and the Network of Microtubule-Associated Functions
Mol Plant,
October 3, 2008;
(2008)
ssn060v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ma, J. Tran, C. Li, L. Ganesan, D. Wood, and N. Morrissette
Secondary Mutations Correct Fitness Defects in Toxoplasma gondii With Dinitroaniline Resistance Mutations
Genetics,
October 1, 2008;
180(2):
845 - 856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Yao, Y. Wakamatsu, T. J. Itoh, T. Shoji, and T. Hashimoto
Arabidopsis SPIRAL2 promotes uninterrupted microtubule growth by suppressing the pause state of microtubule dynamics
J. Cell Sci.,
July 15, 2008;
121(14):
2372 - 2381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Fortunati, S. Piconese, P. Tassone, S. Ferrari, and F. Migliaccio
A new mutant of Arabidopsis disturbed in its roots, right-handed slanting, and gravitropism defines a gene that encodes a heat-shock factor
J. Exp. Bot.,
April 9, 2008;
(2008)
ern047v2.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Kirik, U. Herrmann, C. Parupalli, J. C. Sedbrook, D. W. Ehrhardt, and M. Hulskamp
CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis
J. Cell Sci.,
December 15, 2007;
120(24):
4416 - 4425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Ambrose, T. Shoji, A. M. Kotzer, J. A. Pighin, and G. O. Wasteneys
The Arabidopsis CLASP Gene Encodes a Microtubule-Associated Protein Involved in Cell Expansion and Division
PLANT CELL,
September 1, 2007;
19(9):
2763 - 2775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Korolev, H. Buschmann, J. H. Doonan, and C. W. Lloyd
AtMAP70-5, a divergent member of the MAP70 family of microtubule-associated proteins, is required for anisotropic cell growth in Arabidopsis
J. Cell Sci.,
July 1, 2007;
120(13):
2241 - 2247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishida, Y. Kaneko, M. Iwano, and T. Hashimoto
Helical microtubule arrays in a collection of twisting tubulin mutants of Arabidopsis thaliana
PNAS,
May 15, 2007;
104(20):
8544 - 8549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M. Chilley, S. A. Casson, P. Tarkowski, N. Hawkins, K. L.-C. Wang, P. J. Hussey, M. Beale, J. R. Ecker, G. K. Sandberg, and K. Lindsey
The POLARIS Peptide of Arabidopsis Regulates Auxin Transport and Root Growth via Effects on Ethylene Signaling
PLANT CELL,
November 1, 2006;
18(11):
3058 - 3072.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bartolini and G. G. Gundersen
Generation of noncentrosomal microtubule arrays
J. Cell Sci.,
October 15, 2006;
119(20):
4155 - 4163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Shoji, K. Suzuki, T. Abe, Y. Kaneko, H. Shi, J.-K. Zhu, A. Rus, P. M. Hasegawa, and T. Hashimoto
Salt Stress Affects Cortical Microtubule Organization and Helical Growth in Arabidopsis
Plant Cell Physiol.,
August 1, 2006;
47(8):
1158 - 1168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nakajima, T. Kawamura, and T. Hashimoto
Role of the SPIRAL1 Gene Family in Anisotropic Growth of Arabidopsis thaliana
Plant Cell Physiol.,
April 1, 2006;
47(4):
513 - 522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Chevalier, M. Batoux, L. Fulton, K. Pfister, R. K. Yadav, M. Schellenberg, and K. Schneitz
STRUBBELIG defines a receptor kinase-mediated signaling pathway regulating organ development in Arabidopsis
PNAS,
June 21, 2005;
102(25):
9074 - 9079.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Y.L. Yuen, J. C. Sedbrook, R. M. Perrin, K. L. Carroll, and P. H. Masson
Loss-of-Function Mutations of ROOT HAIR DEFECTIVE3 Suppress Root Waving, Skewing, and Epidermal Cell File Rotation in Arabidopsis
Plant Physiology,
June 1, 2005;
138(2):
701 - 714.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Bisgrove, W. E. Hable, and D. L. Kropf
+TIPs and Microtubule Regulation. The Beginning of the Plus End in Plants
Plant Physiology,
December 1, 2004;
136(4):
3855 - 3863.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. O. Wasteneys and Z. Yang
New Views on the Plant Cytoskeleton
Plant Physiology,
December 1, 2004;
136(4):
3884 - 3891.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Shoji, N. N. Narita, K. Hayashi, J. Asada, T. Hamada, S. Sonobe, K. Nakajima, and T. Hashimoto
Plant-Specific Microtubule-Associated Protein SPIRAL2 Is Required for Anisotropic Growth in Arabidopsis
Plant Physiology,
December 1, 2004;
136(4):
3933 - 3944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Dixit and R. Cyr
The Cortical Microtubule Array: From Dynamics to Organization
PLANT CELL,
October 1, 2004;
16(10):
2546 - 2552.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nakamura, K. Naoi, T. Shoji, and T. Hashimoto
Low Concentrations of Propyzamide and Oryzalin Alter Microtubule Dynamics in Arabidopsis Epidermal Cells
Plant Cell Physiol.,
September 15, 2004;
45(9):
1330 - 1334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Sedbrook, D. W. Ehrhardt, S. E. Fisher, W.-R. Scheible, and C. R. Somerville
The Arabidopsis SKU6/SPIRAL1 Gene Encodes a Plus End-Localized Microtubule-Interacting Protein Involved in Directional Cell Expansion
PLANT CELL,
June 1, 2004;
16(6):
1506 - 1520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
