Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (104)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balk, J.
Right arrow Articles by Leaver, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balk, J.
Right arrow Articles by Leaver, C. J.
Agricola
Right arrow Articles by Balk, J.
Right arrow Articles by Leaver, C. J.
The Plant Cell, Vol. 13, 1803-1818, August 2001, Copyright © 2001,
American Society of Plant Biologists

The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release

Janneke Balk1 and Christopher J. Leaver

University of Oxford, Department of Plant Sciences, South Parks Road, Oxford OX1 3RB, United Kingdom

1 To whom correspondence should be addressed. E-mail janneke.balk{at}plant-sciences.ox.ac.uk; fax 44-1865-275144

In mammals, mitochondria have been shown to play a key intermediary role in apoptosis, a morphologically distinct form of programmed cell death (PCD), for example, through the release of cytochrome c, which activates a proteolytic enzyme cascade, resulting in specific nuclear DNA degradation and cell death. In plants, PCD is a feature of normal development, including the penultimate stage of anther development, leading to dehiscence and pollen release. However, there is little evidence that plant mitochondria are involved in PCD. In a wide range of plant species, anther and/or pollen development is disrupted in a class of mutants termed CMS (for cytoplasmic male sterility), which is associated with mutations in the mitochondrial genome. On the basis of the manifestation of a number of morphological and biochemical markers of apoptosis, we have shown that the PET1-CMS cytoplasm in sunflower causes premature PCD of the tapetal cells, which then extends to other anther tissues. These features included cell condensation, oligonucleosomal cleavage of nuclear DNA, separation of chromatin into delineated masses, and initial persistence of mitochondria. In addition, immunocytochemical analysis revealed that cytochrome c was released partially from the mitochondria into the cytosol of tapetal cells before the gross morphological changes associated with PCD. The decrease in cytochrome c content in mitochondria isolated from male sterile florets preceded a decrease in the integrity of the outer mitochondrial membrane and respiratory control ratio. Our data suggest that plant mitochondria, like mammalian mitochondria, play a key role in the induction of PCD. The tissue-specific nature of the CMS phenotype is discussed with regard to cellular respiratory demand and PCD during normal anther development.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Y. Zhu, T. Saraike, Y. Yamamoto, H. Hagita, S. Takumi, and K. Murai
orf260cra, a Novel Mitochondrial Gene, is Associated with the Homeotic Transformation of Stamens into Pistil-Like Structures (Pistillody) in Alloplasmic Wheat
Plant Cell Physiol., November 1, 2008; 49(11): 1723 - 1733.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. Yang, T. Terachi, and H. Yamagishi
Inhibition of chalcone synthase Expression in Anthers of Raphanus sativus with Ogura Male Sterile Cytoplasm
Ann. Bot., October 1, 2008; 102(4): 483 - 489.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Fujii and K. Toriyama
Genome Barriers between Nuclei and Mitochondria Exemplified by Cytoplasmic Male Sterility
Plant Cell Physiol., October 1, 2008; 49(10): 1484 - 1494.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
U. Mabalirajan, A. K. Dinda, S. Kumar, R. Roshan, P. Gupta, S. K. Sharma, and B. Ghosh
Mitochondrial Structural Changes and Dysfunction Are Associated with Experimental Allergic Asthma
J. Immunol., September 1, 2008; 181(5): 3540 - 3548.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. K. Azad, T. Ishikawa, T. Ishikawa, Y. Sawa, and H. Shibata
Intracellular energy depletion triggers programmed cell death during petal senescence in tulip
J. Exp. Bot., May 31, 2008; (2008) ern066v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Xu, Y. Yang, Z. Zhang, W. Chen, C. Zhang, L. Zhang, S. Zou, and Z. Ma
Expression of the nuclear gene TaFAd is under mitochondrial retrograde regulation in anthers of male sterile wheat plants with timopheevii cytoplasm
J. Exp. Bot., April 4, 2008; (2008) ern068v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Gonzalez-Melendi, M. Uyttewaal, C. N. Morcillo, J. R. Hernandez Mora, S. Fajardo, F. Budar, and M. M. Lucas
A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus)
J. Exp. Bot., March 1, 2008; 59(4): 827 - 838.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. R. Phillips, A. Suttangkakul, and R. D. Vierstra
The ATG12-Conjugating Enzyme ATG10 Is Essential for Autophagic Vesicle Formation in Arabidopsis thaliana
Genetics, March 1, 2008; 178(3): 1339 - 1353.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. J. Reape, E. M. Molony, and P. F. McCabe
Programmed cell death in plants: distinguishing between different modes
J. Exp. Bot., February 5, 2008; (2008) erm258v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Programmed Cell Death in Plants: A Role for Mitochondrial-Associated Hexokinases
PLANT CELL, September 1, 2006; 18(9): 2097 - 2099.
[Full Text] [PDF]

Home page
 J Exp BotHome page
G. Vizcay-Barrena and Z. A. Wilson
Altered tapetal PCD and pollen wall development in the Arabidopsis ms1 mutant.
J. Exp. Bot., August 1, 2006; 57(11): 2709 - 2717.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kawanabe, T. Ariizumi, M. Kawai-Yamada, H. Uchimiya, and K. Toriyama
Abolition of the Tapetum Suicide Program Ruins Microsporogenesis
Plant Cell Physiol., June 1, 2006; 47(6): 784 - 787.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Vacca, D. Valenti, A. Bobba, R. S. Merafina, S. Passarella, and E. Marra
Cytochrome c Is Released in a Reactive Oxygen Species-Dependent Manner and Is Degraded via Caspase-Like Proteases in Tobacco Bright-Yellow 2 Cells en Route to Heat Shock-Induced Cell Death
Plant Physiology, May 1, 2006; 141(1): 208 - 219.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
H. J. ROGERS
Programmed Cell Death in Floral Organs: How and Why do Flowers Die?
Ann. Bot., March 1, 2006; 97(3): 309 - 315.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
N. L. Taylor, J. L. Heazlewood, D. A. Day, and A. H. Millar
Differential Impact of Environmental Stresses on the Pea Mitochondrial Proteome
Mol. Cell. Proteomics, August 1, 2005; 4(8): 1122 - 1133.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. Casolo, E. Petrussa, J. Krajnakova, F. Macri, and A. Vianello
Involvement of the mitochondrial KATP+ channel in H2O2- or NO-induced programmed death of soybean suspension cell cultures
J. Exp. Bot., March 1, 2005; 56(413): 997 - 1006.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
G. C. Pagnussat, H.-J. Yu, Q. A. Ngo, S. Rajani, S. Mayalagu, C. S. Johnson, A. Capron, L.-F. Xie, D. Ye, and V. Sundaresan
Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis
Development, February 1, 2005; 132(3): 603 - 614.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. W. Yang, S. K. Kim, and W. T. Kim
Perturbation of NgTRF1 Expression Induces Apoptosis-Like Cell Death in Tobacco BY-2 Cells and Implicates NgTRF1 in the Control of Telomere Length and Stability
PLANT CELL, December 1, 2004; 16(12): 3370 - 3385.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. R. Hanson and S. Bentolila
Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development
PLANT CELL, June 1, 2004; 16(suppl_1): S154 - S169.
[Full Text] [PDF]

Home page
 Plant CellHome page
N. V. Chichkova, S. H. Kim, E. S. Titova, M. Kalkum, V. S. Morozov, Y. P. Rubtsov, N. O. Kalinina, M. E. Taliansky, and A. B. Vartapetian
A Plant Caspase-Like Protease Activated during the Hypersensitive Response
PLANT CELL, January 1, 2004; 16(1): 157 - 171.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. L. Heazlewood, J. S. Tonti-Filippini, A. M. Gout, D. A. Day, J. Whelan, and A. H. Millar
Experimental Analysis of the Arabidopsis Mitochondrial Proteome Highlights Signaling and Regulatory Components, Provides Assessment of Targeting Prediction Programs, and Indicates Plant-Specific Mitochondrial Proteins
PLANT CELL, January 1, 2004; 16(1): 241 - 256.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Giege, J. L. Heazlewood, U. Roessner-Tunali, A. H. Millar, A. R. Fernie, C. J. Leaver, and L. J. Sweetlove
Enzymes of Glycolysis Are Functionally Associated with the Mitochondrion in Arabidopsis Cells
PLANT CELL, September 1, 2003; 15(9): 2140 - 2151.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Zheng, Q. Xia, M. Dauk, W. Shen, G. Selvaraj, and J. Zou
Arabidopsis AtGPAT1, a Member of the Membrane-Bound Glycerol-3-Phosphate Acyltransferase Gene Family, Is Essential for Tapetum Differentiation and Male Fertility
PLANT CELL, August 1, 2003; 15(8): 1872 - 1887.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
X. Yang, C. A. Makaroff, and H. Ma
The Arabidopsis MALE MEIOCYTE DEATH1 Gene Encodes a PHD-Finger Protein That Is Required for Male Meiosis
PLANT CELL, June 1, 2003; 15(6): 1281 - 1295.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
H. Thomas, H. J. Ougham, C. Wagstaff, and A. D. Stead
Defining senescence and death
J. Exp. Bot., April 1, 2003; 54(385): 1127 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
S. A. Frank and C. M. Barr
Programmed Cell Death and Hybrid Incompatibility
J. Hered., March 1, 2003; 94(2): 181 - 183.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Liu and P. S. Schnable
Functional Specialization of Maize Mitochondrial Aldehyde Dehydrogenases
Plant Physiology, December 1, 2002; 130(4): 1657 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
E. VIROLAINEN, O. BLOKHINA, and K. FAGERSTEDT
Ca2+-induced High Amplitude Swelling and Cytochrome c Release From Wheat (Triticum aestivum L.) Mitochondria Under Anoxic Stress
Ann. Bot., October 1, 2002; 90(4): 509 - 516.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. A. Christensen, S. W. Gorsich, R. H. Brown, L. G. Jones, J. Brown, J. M. Shaw, and G. N. Drews
Mitochondrial GFA2 Is Required for Synergid Cell Death in Arabidopsis
PLANT CELL, September 1, 2002; 14(9): 2215 - 2232.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. A. Robson and G. C. Vanlerberghe
Transgenic Plant Cells Lacking Mitochondrial Alternative Oxidase Have Increased Susceptibility to Mitochondria-Dependent and -Independent Pathways of Programmed Cell Death
Plant Physiology, August 1, 2002; 129(4): 1908 - 1920.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. C. Vanlerberghe, C. A. Robson, and J. Y.H. Yip
Induction of Mitochondrial Alternative Oxidase in Response to a Cell Signal Pathway Down-Regulating the Cytochrome Pathway Prevents Programmed Cell Death
Plant Physiology, August 1, 2002; 129(4): 1829 - 1842.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Arpagaus, A. Rawyler, and R. Braendle
Occurrence and Characteristics of the Mitochondrial Permeability Transition in Plants
J. Biol. Chem., January 11, 2002; 277(3): 1780 - 1787.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists