This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (39) Citing Articles via Google Scholar Google Scholar Articles by Raizada, M. N. Articles by Walbot, V. Search for Related Content PubMed PubMed Citation Articles by Raizada, M. N. Articles by Walbot, V. Agricola Articles by Raizada, M. N. Articles by Walbot, V.

Somatic and Germinal Mobility of the RescueMu Transposon in Transgenic Maize

Department of Biological Sciences, Stanford University, Stanford, California 94305-5020

² To whom correspondence should be addressed. E-mail walbot{at}stanford.edu; fax 650-725-8221

RescueMu, a Mu1 element containing a bacterial plasmid, is mobilized by MuDR in transgenic maize. Somatic excision from a cell-autonomous marker gene yields >90% single cell sectors; empty donor sites often have deletions and insertions, including up to 210 bp of RescueMu/Mu1 terminal DNA. Late somatic insertions are contemporaneous with excisions, suggesting that "cut-and-paste" transposition occurs in the soma. During reproduction, RescueMu transposes infrequently from the initial transgene array, but once transposed, RescueMu is suitable for high throughput gene mutation and cloning. As with MuDR/Mu elements, heritable RescueMu insertions are not associated with excisions. Both somatic and germinal RescueMu insertions occur preferentially into genes and gene-like sequences, but they exhibit weak target site preferences. New insights into Mu behaviors are discussed with reference to two models proposed to explain the alternative outcomes of somatic and germinal events: a switch from somatic cut-and-paste to germinal replicative transposition or to host-mediated gap repair from sister chromatids.

This article has been cited by other articles:

				J. Li, T.-J. Wen, and P. S. Schnable Role of RAD51 in the Repair of MuDR-Induced Double-Strand Breaks in Maize (Zea mays L.) Genetics, January 1, 2008; 178(1): 57 - 66. [Abstract] [Full Text] [PDF]

				W. Yu, F. Han, Z. Gao, J. M. Vega, and J. A. Birchler Construction and behavior of engineered minichromosomes in maize PNAS, May 22, 2007; 104(21): 8924 - 8929. [Abstract] [Full Text] [PDF]

				W. Yu, J. C. Lamb, F. Han, and J. A. Birchler Cytological Visualization of DNA Transposons and Their Transposition Pattern in Somatic Cells of Maize Genetics, January 1, 2007; 175(1): 31 - 39. [Abstract] [Full Text] [PDF]

				P. D. Rabinowicz, R. Citek, M. A. Budiman, A. Nunberg, J. A. Bedell, N. Lakey, A. L. O'Shaughnessy, L. U. Nascimento, W. R. McCombie, and R. A. Martienssen Differential methylation of genes and repeats in land plants Genome Res., October 1, 2005; 15(10): 1431 - 1440. [Abstract] [Full Text] [PDF]

				M. D. Yandeau-Nelson, Q. Zhou, H. Yao, X. Xu, B. J. Nikolau, and P. S. Schnable MuDR Transposase Increases the Frequency of Meiotic Crossovers in the Vicinity of a Mu Insertion in the Maize a1 Gene Genetics, February 1, 2005; 169(2): 917 - 929. [Abstract] [Full Text] [PDF]

				R. M. Dent, C. M. Haglund, B. L. Chin, M. C. Kobayashi, and K. K. Niyogi Functional Genomics of Eukaryotic Photosynthesis Using Insertional Mutagenesis of Chlamydomonas reinhardtii Plant Physiology, February 1, 2005; 137(2): 545 - 556. [Abstract] [Full Text] [PDF]

				N. M. Springer, X. Xu, and W. B. Barbazuk Utility of Different Gene Enrichment Approaches Toward Identifying and Sequencing the Maize Gene Space Plant Physiology, October 1, 2004; 136(2): 3023 - 3033. [Abstract] [Full Text] [PDF]

				Z. Xu, X. Yan, S. Maurais, H. Fu, D. G. O'Brien, J. Mottinger, and H. K. Dooner Jittery, a Mutator Distant Relative with a Paradoxical Mobile Behavior: Excision without Reinsertion PLANT CELL, May 1, 2004; 16(5): 1105 - 1114. [Abstract] [Full Text] [PDF]

				L. E. Palmer, P. D. Rabinowicz, A. L. O'Shaughnessy, V. S. Balija, L. U. Nascimento, S. Dike, M. de la Bastide, R. A. Martienssen, and W. R. McCombie Maize Genome Sequencing by Methylation Filtration Science, December 19, 2003; 302(5653): 2115 - 2117. [Abstract] [Full Text] [PDF]

				S.-H. Kim and V. Walbot Deletion Derivatives of the MuDR Regulatory Transposon of Maize Encode Antisense Transcripts but Are Not Dominant-Negative Regulators of Mutator Activities PLANT CELL, October 1, 2003; 15(10): 2430 - 2447. [Abstract] [Full Text] [PDF]

				B. P. May, H. Liu, E. Vollbrecht, L. Senior, P. D. Rabinowicz, D. Roh, X. Pan, L. Stein, M. Freeling, D. Alexander, et al. Maize-targeted mutagenesis: A knockout resource for maize PNAS, September 30, 2003; 100(20): 11541 - 11546. [Abstract] [Full Text] [PDF]

				F. Chalvet, C. Grimaldi, F. Kaper, T. Langin, and M.-J. Daboussi Hop, an Active Mutator-like Element in the Genome of the Fungus Fusarium oxysporum Mol. Biol. Evol., August 1, 2003; 20(8): 1362 - 1375. [Abstract] [Full Text] [PDF]

				Q. Dong, L. Roy, M. Freeling, V. Walbot, and V. Brendel ZmDB, an integrated database for maize genome research Nucleic Acids Res., January 1, 2003; 31(1): 244 - 247. [Abstract] [Full Text] [PDF]

				C. R. Dietrich, F. Cui, M. L. Packila, J. Li, D. A. Ashlock, B. J. Nikolau, and P. S. Schnable Maize Mu Transposons Are Targeted to the 5' Untranslated Region of the gl8 Gene and Sequences Flanking Mu Target-Site Duplications Exhibit Nonrandom Nucleotide Composition Throughout the Genome Genetics, February 1, 2002; 160(2): 697 - 716. [Abstract] [Full Text] [PDF]