Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (37)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chua, Y. L.
Right arrow Articles by Gray, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chua, Y. L.
Right arrow Articles by Gray, J. C.
Agricola
Right arrow Articles by Chua, Y. L.
Right arrow Articles by Gray, J. C.
Plant Cell, Vol. 13, 599-612, March 2001, Copyright © 2001, American Society of Plant Physiologists

Targeted Histone Acetylation and Altered Nuclease Accessibility over Short Regions of the Pea Plastocyanin Gene

Yii Leng Chua, Anthony P. C. Brown, and John C. Gray
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom

Correspondence to: John C. Gray, jcg2{at}mole.bio.cam.ac.uk (E-mail), 44-1223-333953 (fax)

The chromatin structure of the pea plastocyanin gene (PetE) was examined at three different transcriptional states by investigating the acetylation states of histones H3 and H4 and the nuclease accessibility of the gene in pea roots, etiolated shoots, and green shoots. The acetylation states of histones associated with different regions of PetE were analyzed by chromatin immunoprecipitation with antibodies specific for acetylated or nonacetylated histone H3 or H4 tails, followed by polymerase chain reaction quantification. Comparison of pea tissues indicated that histone hyperacetylation was associated with increased PetE transcription in green shoots. Moreover, hyperacetylation of both histones H3 and H4 was targeted to the enhancer/promoter region in green shoots, suggesting that only specific nucleosomes along the gene were modified. Time-course digestions of nuclei with micrococcal nuclease and DNaseI indicated that the enhancer/promoter region was more resistant to digestion in the inactive gene in pea roots than was the same region in the active gene in shoots, whereas the transcribed region of PetE was digested similarly among the tissues. This finding indicates that transcription is accompanied by changes in the nuclease accessibility of the enhancer/promoter region only. Moreover, these results indicate that the changes in nuclease accessibility are organ specific, whereas histone hyperacetylation is light dependent, and they suggest that changes in nuclease accessibility precede histone hyperacetylation during PetE activation.




This article has been cited by other articles:


Home page
 GeneticsHome page
S. Offermann, B. Dreesen, I. Horst, T. Danker, M. Jaskiewicz, and C. Peterhansel
Developmental and Environmental Signals Induce Distinct Histone Acetylation Profiles on Distal and Proximal Promoter Elements of the C4-Pepc Gene in Maize
Genetics, August 1, 2008; 179(4): 1891 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Guo, J. Zhou, A. A. Elling, J.-B. F. Charron, and X. W. Deng
Histone Modifications and Expression of Light-Regulated Genes in Arabidopsis Are Cooperatively Influenced by Changing Light Conditions
Plant Physiology, August 1, 2008; 147(4): 2070 - 2083.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Casati, M. Campi, F. Chu, N. Suzuki, D. Maltby, S. Guan, A. L. Burlingame, and V. Walbot
Histone Acetylation and Chromatin Remodeling Are Required for UV-B-Dependent Transcriptional Activation of Regulated Genes in Maize
PLANT CELL, April 1, 2008; 20(4): 827 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Tsuji, H. Saika, N. Tsutsumi, A. Hirai, and M. Nakazono
Dynamic and Reversible Changes in Histone H3-Lys4 Methylation and H3 Acetylation Occurring at Submergence-inducible Genes in Rice
Plant Cell Physiol., July 1, 2006; 47(7): 995 - 1003.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Offermann, T. Danker, D. Dreymuller, R. Kalamajka, S. Topsch, K. Weyand, and C. Peterhansel
Illumination Is Necessary and Sufficient to Induce Histone Acetylation Independent of Transcriptional Activity at the C4-Specific Phosphoenolpyruvate Carboxylase Promoter in Maize
Plant Physiology, July 1, 2006; 141(3): 1078 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. L. Chua, S. Channeliere, E. Mott, and J. C. Gray
The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Genes & Dev., September 15, 2005; 19(18): 2245 - 2254.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Bertrand, M. Benhamed, Y.-F. Li, M. Ayadi, G. Lemonnier, J.-P. Renou, M. Delarue, and D.-X. Zhou
Arabidopsis HAF2 Gene Encoding TATA-binding Protein (TBP)-associated Factor TAF1, Is Required to Integrate Light Signals to Regulate Gene Expression and Growth
J. Biol. Chem., January 14, 2005; 280(2): 1465 - 1473.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Turck, A. Zhou, and I. E. Somssich
Stimulus-Dependent, Promoter-Specific Binding of Transcription Factor WRKY1 to Its Native Promoter and the Defense-Related Gene PcPR1-1 in Parsley
PLANT CELL, October 1, 2004; 16(10): 2573 - 2585.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. L. Chua, L. A. Watson, and J. C. Gray
The Transcriptional Enhancer of the Pea Plastocyanin Gene Associates with the Nuclear Matrix and Regulates Gene Expression through Histone Acetylation
PLANT CELL, June 1, 2003; 15(6): 1468 - 1479.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. C. Reyes, L. Hennig, and W. Gruissem
Chromatin-Remodeling and Memory Factors. New Regulators of Plant Development
Plant Physiology, November 1, 2002; 130(3): 1090 - 1101.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2001 by the American Society of Plant Biologists