Plant Cell Journal of Pharmacology and Experimental Therapeutics
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Plant Cell, Vol. 12, 721-738, May 2000, Copyright © 2000, American Society of Plant Physiologists

Transgenic Arabidopsis Plants Expressing a Fungal Cutinase Show Alterations in the Structure and Properties of the Cuticle and Postgenital Organ Fusions

Patrick Siebera, Martine Schordereta, Ulrich Rysera, Antony Buchalaa, Pappachan Kolattukudyb, Jean-Pierre Métrauxa, and Christiane Nawratha
a Department of Biology, Unit of Plant Biology, University of Fribourg, CH-1700 Fribourg, Switzerland
b Departments of Biochemistry and Medical Biochemistry and Neurobiotechnology Center, Ohio State University, Columbus, Ohio 43210

Correspondence to: Christiane Nawrath, Christiane.Nawrath{at}unifr.ch (E-mail), 41-26-300-97-40 (fax)

A major structural component of the cuticle of plants is cutin. Analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. Therefore, transgenic Arabidopsis plants were generated that express and secrete a cutinase from Fusarium solani f sp pisi. Arabidopsis plants expressing the cutinase in the extracellular space showed an altered ultrastructure of the cuticle and an enhanced permeability of the cuticle to solutes. In addition, pollen could germinate on fully differentiated leaves of cutinase-expressing plants but not on control leaves. These differences coincided with strong postgenital organ fusions. The junctions of the fusions contained pectic polysaccharides. As fused organs grew apart from each other, organ deformations and protrusions of epidermal cells developed at positions with high mechanical stress. These results demonstrate that an intact cutin layer not only is important for plant–environment interactions but also prevents fusions between different plant organs and is therefore necessary for normal epidermal differentiation and organ formation.




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